| Reference | [1]. Arch Physiol Biochem. 2018 Oct;124(4):335-343. doi: 10.1080/13813455.2017.1402352. Epub 2017 Nov 20.<br />
Screening of analgesic activity of Tunisian Urtica dioica and analysis of its major bioactive compounds by GCMS.<br />
Dhouibi R(1), Moalla D(1), Ksouda K(1), Ben Salem M(1), Hammami S(1), Sahnoun Z(2), Zeghal KM(1), Affes H(1).<br />
Author information: (1)a Laboratory of Pharmacology, Faculty of Medicine of Sfax , University of Sfax , Sfax , Tunisia. (2)b Research Unit of Pharmacology and Toxicology of Xenobiotics, Faculty of Medicine of Sfax , University of Sfax , Sfax , Tunisia.<br />
The present study was aimed to evaluate the analgesic properties of Urtica dioica (UD) and to profile phytochemicals by gas chromatography-mass spectrometry (GC-MS). The ethanolic extracts were prepared by maceration method and extraction using rotary evaporator. The analgesic activity was analysed by hot plate method, formalin test, acetic acid-induced writhing test and the tail-flick test with different doses of the ethanolic extract. In all tests, the leaf's ethanolic extract exhibited significant analgesic activity (p < .001) at a dose of 400 mg/kg. Even with a low dose, we noticed an analgesic activity with many tests. The GC-MS analysis of the ethanol extract of leaf revealed many compounds; 2-methyltetradecane dodecane, 2,6,11-trimethyl-; 2,6,11-trimethyldodecane, and trimethylhexane which are pharmaceutically the most important. These findings justify that UD can be a valuable natural analgesic source which seemed to provide potential phototherapeutics against various ailments. The analysis of ethanolic extract of UD by GCMS revealed the presence of several compounds including polyphenols, flavonoids, triterpenes which can explain the analgesic effect of UD and its mechanism of action. Hence, UD could be another therapeutic alternative for relieving pain and for minimising the use of drugs that have long-term secondary effects.<br />
DOI: 10.1080/13813455.2017.1402352 PMID: 29157001 [Indexed for MEDLINE]<br />
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[2]. Lunshof, H.J., Van Steenis, J. and Waterman, H.I., 1947.<br />
Preparation and some physical constants of 2‐methyltetradecane and 3‐methylpentadecane.<br />
Abstract: Since the authors were interested in the physical constants of some branched chain hydrocarbons in connection with other work, 2-methyltetradecane and 3-methylpentadecane were prepared and some of their physical constants determined.<br />
Recueil des Travaux Chimiques des Pays‐Bas, 66(6), pp.348-352.<br />
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[3]. Villasenor, I.M., De Ocampo, E.J. and Bremner, J.B., 1995.<br />
Genotoxic acetamide (s) from capsicum frutescens fruits.<br />
Abstract: Extracts from the fruits of Capsicum frutescens Linn. were monitored for their mutagenic activities using the Micronucleus Test. The GC-MSD of the mutagenic fraction, FB4C4, gave 7 peaks. Repeated flash chromatography of FB4C4 gave 2 intractable mixtures, CF-1 and CF-2. CF-2 showed spectral properties comparable to those of natural capsaicin. Spectral analyses of CF-1 suggested that the major component is 3-acetamido-2-methyltetradecane. Results of the Micronucleus Test indicated that CF-1 is mutagenic. Key Words: Capsicum frutescens fruitsmutagensMicronucleus Testacetamide<br />
Natural Product Letters, 6(4), pp.247-253.<br />
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[4]. Benmeddour, T., Laouer, H., Akkal, S. and Flamini, G., 2015.<br />
Chemical composition and antibacterial activity of essential oil of Launaea lanifera Pau grown in Algerian arid steppes.<br />
Abstract: Objective To evaluate the essential oil composition and the antibacterial activity of an Algerian endemic plant, Launaea lanifera Pau (L. lanifera), grown in arid steppe regions. Methods L. lanifera essential oil was isolated from aerial parts by steam distillation and its chemical composition was evaluated by gas chromatography-flame ionization detector and gas chromatography with electron impact mass spectrometry. Furthermore, its in vitro antibacterial activity against four bacterial strains was tested following the agar disk diffusion method. Results This species had a very low essential oil yield (0.005%). Twenty-four (92.6%) individual components were identified. The main constituents were hexahydrofarnesyl acetone (31.6%), (E)-β-ionone (8.5%), (E)-β-damascenone (7.0%), 2-methyltetradecane (3.8%), n-heptadecane (3.8%), limonene (2.8%) and β-caryophyllene (2.8%). No noteworthy antimicrobial activity was observed on the tested bacteria, neither Gram negative nor Gram positive. Conclusions This is the first report on the volatile constituents and antibacterial activity of L. lanifera. The studied essential oil does not possess significant activity against the tested microorganisms.<br />
Asian Pacific Journal of Tropical Biomedicine, 5(11), pp.960-964.<br />
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[5]. Gheybi, S., Asnaashari, S., Moghaddam, S.B., Ebrahimi, A. and Afshar, F.H., 2015.<br />
Volatile components of aerial parts of Delphinium speciosum MB growing in Iran.<br />
Abstract: The chemical composition of the essential oil from aerial parts of Delphinium speciosum (Family: Ranunculaceae) occurring in the Northwest of Iran (collected from Kandovan village, East Azarbaijan province) was analyzed for the first time by GC-FID and GC-MS. A total of 32 components were identified, accounting for 98.25% of the total oil. The major fraction of the volatile oil was represented by linear hydrocarbons (84.22%) with tricosane (29.32%), pentacosane (23.81%) and heneicosane (8.17%) as the main representatives in this fraction. In addition, the oil contained moderate amount of oxygenated compounds (14.03%), with hexahydrofarensyl acetone or phytone as the most abundant representative (8.87%). © 2015 by Kermanshah University of Medical Sciences.<br />
Journal of Reports in Pharmaceutical Sciences, 4(2), pp.191-195.
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