2,4-Dinitrofluorobenzene

• CAT Number: R051809
• CAS Number: 70-34-8
• Molecular Formula: C6H3FN2O4
• Molecular Weight: 186.098
• Purity: ≥95%
• Synonyms: 1-Fluoro-2,4-dinitrobenzene; 2,4-Dinitrobenzene fluoride; 4-Fluoro-1,3-dinitrobenzene; DFB; DNFB; FDNB; Fluorodinitrobenzene; NSC 33519
• Storage: Store at +4C
• IUPAC Name: 1-fluoro-2,4-dinitrobenzene
• InChI: InChI=1S/C6H3FN2O4/c7-5-2-1-4(8(10)11)3-6(5)9(12)13/h1-3H
• InChIKey: LOTKRQAVGJMPNV-UHFFFAOYSA-N
• SMILES: C1=CC(=C(C=C1[N+](=O)[O-])[N+](=O)[O-])F

2,4-Dinitrofluorobenzene, also known as Sanger’s reagent, is a chemical compound widely used in biochemical research, particularly for the identification and labeling of amino acids and peptides. It reacts with free amino groups to form stable dinitrophenyl (DNP) derivatives, allowing for the analysis of amino acid sequences in proteins. This reagent was historically used in sequencing techniques to determine the terminal amino acids of peptides. Its high reactivity, due to the presence of both nitro groups and a fluorine atom, makes it a powerful tool for nucleophilic substitution reactions in organic synthesis as well.