2’,7’-Dichlorodihydrofluorescein Diacetate

• CAT Number: R056013
• CAS Number: 4091-99-0
• Molecular Formula: C24H16Cl2O7
• Molecular Weight: 487.30
• Purity: ≥95%
• Synonyms: 2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic Acid; o-(2,7-Dichloro-3,6-dihydroxyxanthen-9-yl)benzoic Acid Diacetate; 2’,7’-Dichlorodihydrofluorescein Diacetate; DCFH; H2DCFDA
• Target: NF-κB
• Storage: -20°C
• IUPAC Name: 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
• InChI: InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
• InChIKey: PXEZTIWVRVSYOK-UHFFFAOYSA-N
• SMILES: CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl

2’,7’-Dichlorodihydrofluorescein diacetate (Cat No.:R056013) is a chemically modified form of fluorescein used primarily as a fluorescent probe in biological research. This compound is non-fluorescent until it is hydrolyzed intracellularly to 2′,7′-dichlorodihydrofluorescein by cellular esterases and then oxidized by reactive oxygen species (ROS) to form 2’,7’-dichlorofluorescein, a highly fluorescent compound. It is extensively utilized to measure oxidative stress within cells due to its sensitivity to ROS, making it a valuable tool in studies related to aging, cancer, and the effects of various environmental stresses on cell health.