For research use only. Not for therapeutic Use.
3-Methyladenine (Cat No.:I003848) is a widely used inhibitor of autophagy, a cellular process involved in the degradation and recycling of cellular components. It acts by inhibiting the class III phosphoinositide 3-kinase (PI3K) enzyme, specifically Vps34, which is essential for the initiation of autophagy. By blocking autophagy, 3-Methyladenine can affect various cellular processes, including cell survival, differentiation, and development. It has been utilized in both in vitro and in vivo studies to investigate the role of autophagy in various physiological and pathological conditions and to evaluate potential therapeutic strategies targeting autophagy.
| Catalog Number | I003848 |
| CAS Number | 5142-23-4 |
| Synonyms | 3-methylpurin-6-amine |
| Molecular Formula | C₆H₇N₅ |
| Purity | ≥95% |
| Target | Autophagy |
| Solubility | DMSO: ≥ 1.5 mg/mL, DMSO: ＜ 6.8 mg/mL, H2O：≤ 8.82 mg/mL (Need ultrasonic) |
| Storage | 3 years -20C powder |
| IC50 | 60 μM |
| IUPAC Name | 3-methyl-7H-purin-6-imine |
| InChI | InChI=1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3,7H,1H3,(H,8,9) |
| InChIKey | ZPBYVFQJHWLTFB-UHFFFAOYSA-N |
| SMILES | CN1C=NC(=N)C2=C1N=CN2 |
| Reference | </br>1:Role of autophagy in the progression of osteoarthritis: The autophagy inhibitor, 3-methyladenine, aggravates the severity of experimental osteoarthritis. Cheng NT, Meng H, Ma LF, Zhang L, Yu HM, Wang ZZ, Guo A.Int J Mol Med. 2017 May;39(5):1224-1232. doi: 10.3892/ijmm.2017.2934. Epub 2017 Mar 23. PMID: 28339018 Free PMC Article</br>2:Autophagy inhibitor 3-methyladenine alleviates overload-exercise-induced cardiac injury in rats. Liu H, Lei H, Shi Y, Wang JJ, Chen N, Li ZH, Chen YF, Ye QF, Yang Y.Acta Pharmacol Sin. 2017 Mar 6. doi: 10.1038/aps.2016.169. [Epub ahead of print] PMID: 28260802 </br>3:Acanthopanax <i>versus</i> 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats. Wang X, Zhou G, Liu C, Wei R, Zhu S, Xu Y, Wu M, Miao Q.Mediators Inflamm. 2016;2016:8369704. doi: 10.1155/2016/8369704. Epub 2016 Dec 28. PMID: 28115794 Free PMC Article</br>4:Biomarkers of cigarette smoking and DNA methylating agents: Raman, SERS and DFT study of 3-methyladenine and 7-methyladenine. Harroun SG, Zhang Y, Chen TH, Ku CR, Chang HT.Spectrochim Acta A Mol Biomol Spectrosc. 2017 Apr 5;176:1-7. doi: 10.1016/j.saa.2016.12.012. Epub 2016 Dec 15. PMID: 28061366 </br>5:Systemic application of 3-methyladenine markedly inhibited atherosclerotic lesion in ApoE<sup>-/-</sup> mice by modulating autophagy, foam cell formation and immune-negative molecules. Dai S, Wang B, Li W, Wang L, Song X, Guo C, Li Y, Liu F, Zhu F, Wang Q, Wang X, Shi Y, Wang J, Zhao W, Zhang L.Cell Death Dis. 2016 Dec 1;7(12):e2498. doi: 10.1038/cddis.2016.376. PMID: 27906187 Free PMC Article</br>6:Characterization of substrate binding and enzymatic removal of a 3-methyladenine lesion from genomic DNA with TAG of MDR A. baumannii. Tomar JS, Narwal M, Kumar P, Peddinti RK.Mol Biosyst. 2016 Oct 18;12(11):3259-3265. PMID: 27714027 </br>7:Intrathecal Injection of 3-Methyladenine Reduces Neuronal Damage and Promotes Functional Recovery via Autophagy Attenuation after Spinal Cord Ischemia/Reperfusion Injury in Rats. Wei X, Zhou Z, Li L, Gu J, Wang C, Xu F, Dong Q, Zhou X.Biol Pharm Bull. 2016;39(5):665-73. doi: 10.1248/bpb.b15-00610. PMID: 27150140 Free Article</br>8:[Autophagy inhibitor 3-methyladenine enhances the sensitivity of nasopharyngeal carcinoma cells to chemotherapy and radiotherapy]. Song L, Ma L, Chen G, Huang Y, Sun X, Jiang C, Liu H.Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2016 Jan;41(1):9-18. doi: 10.11817/j.issn.1672-7347.2016.01.002. Chinese. PMID: 26819419 Free Article</br>9:Low concentrations of chloroquine and 3-methyladenine suppress the viability of retinoblastoma cells synergistically with vincristine independent of autophagy inhibition. Zheng XY, Li LJ, Li W, Jiang PF, Shen HQ, Chen YH, Chen X.Graefes Arch Clin Exp Ophthalmol. 2015 Dec;253(12):2309-15. doi: 10.1007/s00417-015-3157-1. Epub 2015 Sep 3. PMID: 26335535 </br>10:Enhancement of oxaliplatin-induced cell apoptosis and tumor suppression by 3-methyladenine in colon cancer. Tan S, Peng X, Peng W, Zhao Y, Wei Y.Oncol Lett. 2015 May;9(5):2056-2062. Epub 2015 Feb 27. PMID: 26137012 Free PMC Article |
