3MB-PP1

• CAT Number: R008559
• CAS Number: 956025-83-5
• Molecular Formula: C17H21N5
• Molecular Weight: 295.39
• Purity: ≥95%
• Synonyms: 3MB-PP1; 1-(1,1-Dimethylethyl)-3-[(3-methylphenyl)methyl]-1H-Pyrazolo[3,4-d]pyrimidin-4-?amine; 3-MB-PP1
• Target: PLK
• Solubility: Soluble in DMSO
• Storage: Store at -20°C
• IUPAC Name: 1-tert-butyl-3-[(3-methylphenyl)methyl]pyrazolo[3,4-d]pyrimidin-4-amine
• InChI: InChI=1S/C17H21N5/c1-11-6-5-7-12(8-11)9-13-14-15(18)19-10-20-16(14)22(21-13)17(2,3)4/h5-8,10H,9H2,1-4H3,(H2,18,19,20)
• InChIKey: FYCOTGCSHZKHPR-UHFFFAOYSA-N
• SMILES: CC1=CC(=CC=C1)CC2=NN(C3=C2C(=NC=N3)N)C(C)(C)C

3MB-PP1（Cat No.:R008559）is a highly selective and potent small-molecule inhibitor of engineered kinases, particularly used in studies involving analog-sensitive kinase mutants. It specifically inhibits these mutant kinases without affecting the wild-type versions, allowing researchers to precisely control kinase activity in cells. 3MB-PP1 has been widely used in signal transduction research to investigate the roles of kinases in various cellular processes, including cell division, differentiation, and apoptosis. Its high specificity makes it a valuable tool in studying kinase signaling pathways and developing targeted therapies for diseases like cancer.

Reference

1. Cell Cycle. 2011 Oct 15;10(20):3527-32. doi: 10.4161/cc.10.20.17792. Epub 2011
Oct 15.

Generation of a set of conditional analog-sensitive alleles of essential protein
kinases in the fission yeast Schizosaccharomyces pombe.

Cipak L(1), Zhang C, Kovacikova I, Rumpf C, Miadokova E, Shokat KM, Gregan J.

Author information:
(1)Max F Perutz Laboratories, University of Vienna, Vienna, Austria.

The genome of the fission yeast Schizosaccharomyces pombe encodes for 17 protein
kinases that are essential for viability. Studies of the essential kinases often
require the use of mutant strains carrying conditional alleles. To inactivate
these kinases conditionally, we applied a recently developed chemical genetic
strategy. The mutation of a single residue in the ATP-binding pocket confers
sensitivity to small-molecule inhibitors, allowing for specific inactivation of
the modified kinase. Using this approach, we constructed conditional
analog-sensitive alleles of 13 essential protein kinases in the fission yeast S.
pombe.