3x DYKDDDDK Tag

• CAT Number: I044185
• CAS Number: 921765-76-6
• Molecular Formula: C123H176N30O58
• Molecular Weight: 3002.88
• Purity: ≥95%

3x DYKDDDDK Tag is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag) for purifying difficult proteins that accumulate in low abundance [1][2].
HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)[2].

Reference

[1]. Mishra V. Affinity Tags for Protein Purification. Curr Protein Pept Sci. 2020;21(8):821-830.
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[2]. Abbott JA, et al. The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity. Biochemistry. 2017;56(28):3619-3631.
 [Content Brief]