4-Di-10-ASP

• CAT Number: R071801
• CAS Number: 95378-73-7
• Molecular Formula: C34H55IN2
• Molecular Weight: 618.72
• Purity: ≥95%
• Synonyms: N,N-didecyl-4-[(E)-2-(1-methylpyridin-1-ium-4-yl)ethenyl]aniline;iodide
• InChI: InChI=1S/C34H55N2.HI/c1-4-6-8-10-12-14-16-18-28-36(29-19-17-15-13-11-9-7-5-2)34-24-22-32(23-25-34)20-21-33-26-30-35(3)31-27-33;/h20-27,30-31H,4-19,28-29H2,1-3H3;1H/q+1;/p-1
• InChIKey: PPAYPDQCRKDOKR-UHFFFAOYSA-M
• SMILES: CCCCCCCCCCN(CCCCCCCCCC)C1=CC=C(C=C1)C=CC2=CC=[N+](C=C2)C.[I-]

4-Di-10-ASP is a fluorescent lipophilic tracer (Excitation 485 nm; Emission 620 nm). 4-Di-10-ASP can be used to stain phospholipid membranes in a specific manner[1][2].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. 4-Di-10-ASP (1 μM), DOPC (HY-113424A) (10 mM), and DOPG (HY-142980) (1 mM) is dissolved in methanol/chloroform (25 L, 1:2 v/v).
2. The solution is allowed to dry overnight under vacuum to obtain lamellar lipid films, which in turn are hydrated with the transcription/translation solution (25 L) for three hours at 37℃.
3. An aliquot (10 L) of the solution thus prepared is placed on a glass slide and sealed by a cover glass.
4. The sample is immediately observed with a confocal laser-scanning microscope, an argon laser (488 nm) is employed to excite the 4-Di-10-ASP[2].

Reference

[1]. Z J Huang, et al. Partition coefficients of fluorescent probes with phospholipid membranes. Biochem Biophys Res Commun. 1991 Nov 27;181(1):166-71.
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[2]. Shin-ichiro M Nomura, et al. Gene expression within cell-sized lipid vesicles. Chembiochem. 2003 Nov 7;4(11):1172-5.
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