4-Hydroxymephenytoin

• CAT Number: R001463
• CAS Number: 61837-65-8
• Molecular Formula: C12H14N2O3
• Molecular Weight: 234.25
• Purity: ≥95%
• Synonyms: 5-ethyl-5-(4-hydroxyphenyl)-3-methylimidazolidine-2,4-dione
• InChI: InChI=1S/C12H14N2O3/c1-3-12(8-4-6-9(15)7-5-8)10(16)14(2)11(17)13-12/h4-7,15H,3H2,1-2H3,(H,13,17)
• InChIKey: OQPLORUDZLXXPD-UHFFFAOYSA-N
• SMILES: CCC1(C(=O)N(C(=O)N1)C)C2=CC=C(C=C2)O

4-Hydroxymephenytoin is a metabolism of an antiepileptic agent mephenytoin, which is used as a CYP2C19 substrate.
4-Hydroxymephenytoin is a metabolism of mephenytoin, which is an antiepileptic drug, and used as a CYP2C19 substrate[1]. 4-Hydroxymephenytoin (4′-Hydroxymephenytoin) is used for developing UltraPerformance LC (UPLC) tandem mass spectrometry assays for evaluating CYP probe drugs and their relevant metabolites in human urine or plasma samples[2].

Reference

[1]. Tanaka M, et al. Simple and selective assay of 4-hydroxymephenytoin in human urine using solid-phase extraction and high-performance liquid chromatography with electrochemical detection and its preliminary application to phenotyping test. J Chromatogr B Biomed Appl. 1996 Feb 9;676(1):87-94.
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[2]. Stewart NA, et al. A UPLC-MS/MS assay of the “Pittsburgh cocktail”: six CYP probe-drug/metabolites from human plasma and urine using stable isotope dilution. Analyst. 2011 Feb 7;136(3):605-12.
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