4-Methylumbelliferyl β-D-Glucuronide

• CAT Number: R000631
• CAS Number: 6160-80-1
• Molecular Formula: C16H16O9
• Molecular Weight: 352.295
• Purity: ≥95%
• Synonyms: 4-Methylumbelliferone β-D-Glucuronide; 4-Methyl-2-oxo-2H-1-benzopyran-7-yl β-D-Glucopyranosiduronic Acid; MUG
• Storage: -20°C
• IUPAC Name: (2S,3S,4S,5R,6S)-3,4,5-trihydroxy-6-(4-methyl-2-oxochromen-7-yl)oxyoxane-2-carboxylic acid
• InChI: InChI=1S/C16H16O9/c1-6-4-10(17)24-9-5-7(2-3-8(6)9)23-16-13(20)11(18)12(19)14(25-16)15(21)22/h2-5,11-14,16,18-20H,1H3,(H,21,22)/t11-,12-,13+,14-,16+/m0/s1
• InChIKey: ARQXEQLMMNGFDU-JHZZJYKESA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)OC3C(C(C(C(O3)C(=O)O)O)O)O

4-Methylumbelliferyl β-D-Glucuronide (CAS 6160-80-1)  is a fluorescent substrate of β-glucuronidase. Once cleaved, MUG yields blue fluorescence seen under UV light. It is ideal for working with E.coli and plant molecular studies.

Reference

1.Zhou, Mingjie, et al. "A fluorogenic substrate for β-glucuronidase: applications in fluorometric, polyacrylamide gel and histochemical assays." Journal of biochemical and biophysical methods 33.3 (1996): 197-205.
2.Wu, Xiaojun, D. Loganathan, and Robert J. Linhardt. "Sensitive method for the quantification of β-glucuronidase activity in human urine using capillary electrophoresis with fluorescence detection." Journal of Chromatography B: Biomedical Sciences and Applications 708.1-2 (1998): 61-66.
3.Sarhan, H. R., and H. A. Foster. "A rapid fluorogenic method for the detection of Escherichia coli by the production of β‐glucuronidase." Journal of applied bacteriology 70.5 (1991): 394-400.