4-Methylumbelliferyl α-D-Galactopyranoside

• CAT Number: R000791
• CAS Number: 38597-12-5
• Molecular Formula: C16H18O8
• Molecular Weight: 338.312
• Purity: ≥95%
• Synonyms: 7-(α-D-Galactopyranosyloxy)-4-methyl-2H-1-benzopyran-2-one; 4-Methylumbelliferone α-D-Galactopyranoside; 4-Methylumbelliferyl α-D-Galactoside
• Storage: -20°C
• IUPAC Name: 4-methyl-7-[(2R,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-2-one
• InChI: InChI=1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16+/m1/s1
• InChIKey: YUDPTGPSBJVHCN-CHUNWDLHSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)OC3C(C(C(C(O3)CO)O)O)O

4-Methylum<wbr></wbr>belliferyl-α-<wbr></wbr>D-galacto<wbr></wbr>pyranoside (4-MU-α-Gal) is a fluorogenic substrate of α-<wbr></wbr>galactosidase. In addition to its use in characterizing novel α-<wbr></wbr>galactosidases, 4-MU-α-Gal is used in assays to evaluate deficiency in α-<wbr></wbr>galactosidase activity related to Fabry disease. Hydrolysis of 4-MU-α-Gal releases the fluorescent product 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.