4-Methylumbelliferyl-β-D-Glucuronide (hydrate)

• CAT Number: R065485
• Molecular Formula: C16H16O9 • 2H2O
• Molecular Weight: 388.3
• Purity: ≥95%
• Synonyms: 4-Methylumbelliferyl-β-D-Glucopyranosiduronic Acid;MUG
• Storage: -20°C
• InChI: InChI=1S/C16H16O9.2H2O/c1-6-4-10(17)24-9-5-7(2-3-8(6)9)23-16-13(20)11(18)12(19)14(25-16)15(21)22;;/h2-5,11-14,16,18-20H,1H3,(H,21,22);2*1H2/t11-,12-,13+,14-,16+;;/m0../s1
• InChIKey: WRVAYRGWFCRSBR-CMNHCFHDSA-N
• SMILES: O[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](C(O)=O)[C@@H](O)[C@@H]1O.O.O

4-Methylumbelliferyl-β-D-glucuronide is a fluorogenic substrate of β-glucuronidase. Upon cleavage, this compound yields a blue fluorescent end product that can be monitored by UV light. 4-Methylumbelliferyl-β-D-glucuronide is typically used in the β-glucuronidase reporter system, also known as GUS reporter system, for fluorescent detection of β-glucuronidase gene expression in E. coli and transformed plants.

Reference

1.Jefferson, R.A.,Kavanagh, T.A., and Bevan, M.W. GUS fusions: β-Glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO Journal 6(13), 3901-3907 (1987).