4-Methylumbelliferyl Phosphate

• CAT Number: R065310
• CAS Number: 3368-04-5
• Molecular Formula: C10H9O6P
• Molecular Weight: 256.2
• Purity: ≥95%
• Synonyms: 7-hydroxy-4-Methylcoumarin Phosphate;4-Methylumbelliferone Phosphate;4-MUP;4-MU Phosphate
• Target: Phosphatase
• Storage: -20°C
• InChI: InChI=1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
• InChIKey: BCHIXGBGRHLSBE-UHFFFAOYSA-N
• SMILES: O=C1C=C(C)C2=C(C=C(OP(O)(O)=O)C=C2)O1

4-Methylumbelliferyl Phosphate (4-MUP) is a fluorogenic substrate for phosphatases, including acid and alkaline phosphatases. It is converted to the fluorescent product 4-methylumbelliferone (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.

Reference

1.Fernley, H.N., and Walker, P.G. Kinetic behaviour of calf-intestinal alkaline phosphatase with 4-methylumbelliferyl phosphate. Biochem. J. 97(1), 95-103 (1965).