5(6)-Carboxyfluorescein 3’,6’-Diacetate N-Succinimidyl Ester

• CAT Number: R055453
• CAS Number: 150347-59-4
• Molecular Formula: C29H19NO11
• Molecular Weight: 557.5
• Purity: ≥95%
• Synonyms: 3’,6’-Bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9’-[9H]xanthene]-ar-carboxylic Acid 2,5-Dioxo-1-pyrrolidinyl Ester; 1-[[[3’,6’-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9’-[9H]xanthen]-5(or 6)-yl]carbonyl]oxy]-2,5-pyrrolidinedione; 5(6)-Carb
• Storage: -20°C
• InChIKey: LZFDYQHOQDFMOA-UHFFFAOYSA-N

5(6)-<wbr></wbr>CFDA-SE is a stable, cell-<wbr></wbr>permeable dye that consists of a fluorescein molecule containing two acetate moieties and a SE functional group. Upon diffusion into the intracellular environment, the acetate groups are removed by cellular esterases, rendering the dye highly fluorescent and no longer permeant to the cell membrane. CFDA-SE is often employed to assay cell proliferation as it is partitioned with high fidelity between daughter cells for up to eight generational divisions.