5(6)-ROX

• CAT Number: I013818
• CAS Number: 198978-94-8
• Molecular Formula: C₃₃H₃₀N₂O₅
• Molecular Weight: 534.6
• Purity: ≥95%
• Target: DNA Stain
• Solubility: DMSO
• Storage: -20°C
• IUPAC Name: 1-oxospiro[2-benzofuran-3,16'-3-oxa-9,23-diazaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1(27),2(17),4(15),5(28),13,18-hexaene]-5-carboxylic acid
• InChI: InChI=1S/C33H30N2O5/c36-31(37)20-9-10-21-24(17-20)33(40-32(21)38)25-15-18-5-1-11-34-13-3-7-22(27(18)34)29(25)39-30-23-8-4-14-35-12-2-6-19(28(23)35)16-26(30)33/h9-10,15-17H,1-8,11-14H2,(H,36,37)
• InChIKey: DAKRRPZULHQSKP-UHFFFAOYSA-N
• SMILES: C1CC2=CC3=C(C4=C2N(C1)CCC4)OC5=C(C36C7=C(C=CC(=C7)C(=O)O)C(=O)O6)C=C8CCCN9C8=C5CCC9

5(6)-ROX (Cat No.: I013818) is a fluorescent label commonly employed in molecular biology and genetics research, particularly in real-time polymerase chain reactions (PCR). This dye serves as a reference or passive reference dye in qPCR assays, aiding in the normalization and quantification of target nucleic acids. By emitting fluorescence at a known wavelength, it facilitates accurate and reliable measurement of the amplification process, allowing researchers to monitor and analyze gene expression levels or other nucleic acid-based reactions with high precision. 5(6)-ROX plays a crucial role in ensuring the accuracy of real-time PCR experiments. If you need to, please contact us in time, and we will reply to you as soon as possible within 24 hours.

Reference

[1]. Wang G, et al. Optimization of 6-carboxy-X-rhodamine concentration for real-time polymerase chain reaction using molecular beacon chemistry. Can J Microbiol. 2007 Mar;53(3):391-7.