5-BrdU

• CAT Number: I004215
• CAS Number: 59-14-3
• Molecular Formula: C9H11BrN2O5
• Molecular Weight: 307.1
• Purity: ≥95%
• Synonyms: 5-bromo-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione
• Target: Nucleoside Antimetabolite/Analogue
• Solubility: DMSO: ≥ 41 mg/mL
• Storage: Store at -20C
• IUPAC Name: 5-bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione
• InChI: InChI=1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
• InChIKey: WOVKYSAHUYNSMH-RRKCRQDMSA-N
• SMILES: C1C(C(OC1N2C=C(C(=O)NC2=O)Br)CO)O

5-BrdU(CAT: I004215), also known as 5-bromo-2′-deoxyuridine, is a synthetic nucleoside analogue of thymidine. It is commonly used as a labeling reagent in molecular biology and cell biology research to measure DNA synthesis and cell proliferation. 5-BrdU is incorporated into newly synthesized DNA during the S-phase of the cell cycle, replacing thymidine. Detection of 5-BrdU incorporation allows researchers to visualize and quantify actively dividing cells. It can be detected using various techniques such as immunohistochemistry, immunofluorescence, or flow cytometry. 5-BrdU has been instrumental in studying cell kinetics, DNA replication, and cell proliferation in both normal and disease states.

Reference

1.Barker, Jennifer M., et al. "A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary." PloS one 8.5 (2013): e63692.
2.Buck, Suzanne B., et al. "Detection of S-phase cell cycle progression using 5-ethynyl-2′-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2′-deoxyuridine antibodies." Biotechniques 44.7 (2008): 927-929.
3.Magavi, Sanjay S., and Jeffrey D. Macklis. "Identification of newborn cells by BrdU labeling and immunocytochemistry in vivo." Neural Stem Cells. Humana Press, 2008. 335-343.