For research use only. Not for therapeutic Use.
5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell[1].
| Catalog Number | M023704 |
| CAS Number | 124412-00-6 |
| Synonyms | acetyloxymethyl 3′,6′-diacetyloxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-carboxylate |
| Molecular Formula | C28H20O11 |
| Purity | ≥95% |
| InChI | InChI=1S/C28H20O11/c1-14(29)34-13-35-26(32)17-4-7-21-20(10-17)27(33)39-28(21)22-8-5-18(36-15(2)30)11-24(22)38-25-12-19(37-16(3)31)6-9-23(25)28/h4-12H,13H2,1-3H3 |
| InChIKey | YDBUJZYYYBVSEF-UHFFFAOYSA-N |
| SMILES | CC(=O)OCOC(=O)C1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)OC(=O)C)OC5=C3C=CC(=C5)OC(=O)C)OC2=O |
| Reference | [1]. Elena Gorokhova, et al. A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy. J Microbiol Methods. 2012 Jun;89(3):216-21. |
