5-CFDA-AM

• CAT Number: M023704
• CAS Number: 124412-00-6
• Molecular Formula: C28H20O11
• Molecular Weight: 532.45
• Purity: ≥95%
• Synonyms: acetyloxymethyl 3′,6′-diacetyloxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-carboxylate
• IUPAC Name: acetyloxymethyl 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
• InChI: InChI=1S/C28H20O11/c1-14(29)34-13-35-26(32)17-4-7-21-20(10-17)27(33)39-28(21)22-8-5-18(36-15(2)30)11-24(22)38-25-12-19(37-16(3)31)6-9-23(25)28/h4-12H,13H2,1-3H3
• InChIKey: YDBUJZYYYBVSEF-UHFFFAOYSA-N
• SMILES: CC(=O)OCOC(=O)C1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)OC(=O)C)OC5=C3C=CC(=C5)OC(=O)C)OC2=O

5-CFDA-AM (Cat No.:M023704) is a cell-permeable fluorescent dye commonly used in cellular and molecular biology research. It is an esterified form of carboxyfluorescein that, once inside the cell, is hydrolyzed by intracellular esterases to release the fluorescent carboxyfluorescein. This process makes it useful for monitoring cellular uptake, viability, and metabolic activity, as well as for tracking cellular proliferation and migration. 5-CFDA-AM is widely employed in assays such as flow cytometry, live-cell imaging, and drug screening to assess cellular functions and responses to treatments.