5-FAM-Alkyne

• CAT Number: I015161
• CAS Number: 510758-19-7
• Molecular Formula: C₂₄H₁₅NO₆
• Molecular Weight: 413.38
• Purity: ≥95%
• Target: Phosphatase
• Storage: -20°C
• IUPAC Name: 3',6'-dihydroxy-3-oxo-N-prop-2-ynylspiro[2-benzofuran-1,9'-xanthene]-5-carboxamide
• InChI: InChI=1S/C24H15NO6/c1-2-9-25-22(28)13-3-6-17-16(10-13)23(29)31-24(17)18-7-4-14(26)11-20(18)30-21-12-15(27)5-8-19(21)24/h1,3-8,10-12,26-27H,9H2,(H,25,28)
• InChIKey: UNBAJBIGCBVWJU-UHFFFAOYSA-N
• SMILES: C#CCNC(=O)C1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O)OC2=O

5-FAM-Alkyne（Cat No.:I015161） is a fluorescent biosensor designed specifically for the detection of alkaline phosphatase (ALP) activity. It exhibits high selectivity and sensitivity towards ALP, enabling accurate and reliable measurement of its activity. The fluorophore 5-FAM, combined with the alkyne functional group, allows for efficient detection and quantification of ALP in biological samples. This biosensor can be employed in various applications, including enzymatic assays, imaging studies, and monitoring ALP-related processes in biological systems. Its high specificity and sensitivity make it a valuable tool for ALP detection and analysis.

Reference

[1]. Mengmeng Zhao, et al. A Sensitive Fluorescence Biosensor for Alkaline Phosphatase Activity Based on the Cu(II)-dependent DNAzyme. Anal Chim Acta. 2016 Dec 15;948:98-103.