For research use only. Not for therapeutic Use.
5-Iodoacetamidofluorescein (5-IAF) (Cat.No:I013116) is a fluorescent probe that can be used to label cysteine residues in proteins for detection and purification. It reacts specifically with free cysteine thiol groups, making it a useful tool for studying protein structure and function. 5-IAF is also used in biological imaging to visualize cysteine-rich proteins or molecules.
| Catalog Number | I013116 |
| CAS Number | 63368-54-7 |
| Molecular Formula | C₂₂H₁₄INO₆ |
| Purity | ≥95% |
| Target | Dye Reagents |
| Solubility | DMSO |
| IUPAC Name | N-(3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-yl)-2-iodoacetamide |
| InChI | InChI=1S/C22H14INO6/c23-10-20(27)24-11-1-4-15-14(7-11)21(28)30-22(15)16-5-2-12(25)8-18(16)29-19-9-13(26)3-6-17(19)22/h1-9,25-26H,10H2,(H,24,27) |
| InChIKey | UATCLPJEZJKNHE-UHFFFAOYSA-N |
| SMILES | C1=CC2=C(C=C1NC(=O)CI)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O |
| Reference | [1]. Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013; 8(7): e70374.<br>[2]. Yan Wang, et al. Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2009 Apr 17;1216(16):3533-7. |
