6-CHLORO-3-INDOLYL-BETA-D-GLUCURONIDE CYCLOHEXYLAMMONIUM SALT

• CAT Number: M112837
• CAS Number: 138182-20-4
• Molecular Formula: C20H27ClN2O7
• Molecular Weight: 442.893
• Purity: ≥95%
• Storage: -20°C
• IUPAC Name: (2S,3S,4S,5R,6S)-6-[(6-chloro-1H-indol-3-yl)oxy]-3,4,5-trihydroxyoxane-2-carboxylic acid;cyclohexanamine
• InChI: InChI=1S/C14H14ClNO7.C6H13N/c15-5-1-2-6-7(3-5)16-4-8(6)22-14-11(19)9(17)10(18)12(23-14)13(20)21;7-6-4-2-1-3-5-6/h1-4,9-12,14,16-19H,(H,20,21);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1
• InChIKey: CGXWVPSZSVEFAI-CYRSAHDMSA-N
• SMILES: C1CCC(CC1)N.C1=CC2=C(C=C1Cl)NC=C2OC3C(C(C(C(O3)C(=O)O)O)O)O

6-CHLORO-3-INDOLYL-BETA-D-GLUCURONIDE CYCLOHEXYLAMMONIUM SALT (CAS 138182-20-4) is the cyclohexylammonium salt of 6-Chloro-3-indolyl-β-D-glucuronide, which is a chromogenic substrate for β-glucuronidase. Upon cleavage by β-glucuronidase, an insoluble salmon precipitate is formed that can be used in histochemical analysis of β-glucuronidase activity.

Reference

1.Wozniak, Chris A., and Lowell D. Owens. "Hydrolysis of glucuronide‐based substrates mediated by tungsten, Cu2+, Fe2+, and Zn2+." Physiologia Plantarum 96.3 (1996): 484-490.