7-Hydroxycoumarinyl arachidonate

• CAT Number: M043251
• CAS Number: 161180-11-6
• Molecular Formula: C29H36O4
• Molecular Weight: 448.59
• Purity: ≥95%
• Synonyms: (2-oxochromen-7-yl) (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
• InChI: InChI=1S/C29H36O4/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-28(30)32-26-22-20-25-21-23-29(31)33-27(25)24-26/h6-7,9-10,12-13,15-16,20-24H,2-5,8,11,14,17-19H2,1H3/b7-6-,10-9-,13-12-,16-15-
• InChIKey: SFTGFGOBCQCZDY-DOFZRALJSA-N
• SMILES: CCCCCC=CCC=CCC=CCC=CCCCC(=O)OC1=CC2=C(C=C1)C=CC(=O)O2

7-Hydroxycoumarinyl arachidonate (Cat No.: M043251) is a synthetic fluorogenic substrate designed for studying phospholipase A2 (PLA2) activity. Upon enzymatic hydrolysis, it releases 7-hydroxycoumarin, a fluorescent product that enables sensitive, real-time detection of PLA2-mediated reactions. This substrate is widely used in biochemical assays, drug discovery, and enzymology to investigate lipid metabolism, inflammatory pathways, and enzyme inhibitors. Its strong signal-to-noise ratio and compatibility with high-throughput screening make it a valuable tool for exploring PLA2 function, lipid signaling, and therapeutic target validation.

Reference

[1]. Yuren Wang, et al. A fluorescence-based assay for monoacylglycerol lipase compatible with inhibitor screening. Assay Drug Dev Technol. 2008 Jun;6(3):387-93.
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[2]. Z Huang, et al. A continuous fluorescence-based assay for the human high-molecular-weight cytosolic phospholipase A2. Anal Biochem. 1994 Oct;222(1):110-5.
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[3]. R T Pickard, et al. Identification of essential residues for the catalytic function of 85-kDa cytosolic phospholipase A2. Probing the role of histidine, aspartic acid, cysteine, and arginine. J Biol Chem. 1996 Aug 9;271(32):19225-31.
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