9-[4-[Bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]am

• CAT Number: R060276
• CAS Number: 145037-81-6
• Molecular Formula: C52H59BrN4O19
• Molecular Weight: 1123.957
• Purity: ≥95%
• Synonyms: Rhod 2AM
• Storage: -20°C
• IUPAC Name: bis[2-(acetyloxymethoxy)-2-oxoethyl]-[2-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-4-[3,6-bis(dimethylamino)xanthen-9-ylidene]cyclohexa-2,5-dien-1-ylidene]azanium;bromide
• InChI: InChI=1S/C52H59N4O19.BrH/c1-32-10-16-42(55(24-48(61)71-28-67-33(2)57)25-49(62)72-29-68-34(3)58)46(20-32)65-18-19-66-47-21-37(11-17-43(47)56(26-50(63)73-30-69-35(4)59)27-51(64)74-31-70-36(5)60)52-40-14-12-38(53(6)7)22-44(40)75-45-23-39(54(8)9)13-15-41(45)52;/h10-17,20-23H,18-19,24-31H2,1-9H3;1H/q+1;/p-1
• InChIKey: RWXWZOWDWYQKBK-UHFFFAOYSA-M
• SMILES: CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=CC(=C3C4=C(C=C(C=C4)N(C)C)OC5=C3C=CC(=C5)N(C)C)C=CC2=[N+](CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C.[Br-]

Rhod-2 AM is an acetoxymethyl (AM) ester of the red fluorescent calcium indicator rhod-2. The AM group facilitates cellular uptake and is removed by cytoplasmic esterases, resulting in intracellular accumulation of rhod-2. Rhod-2 AM selectively accumulates within mitochondria and, as a result, is commonly used to monitor calcium changes within this organelle. Excitation and emission maxima are 557 and 581 nm, respectively.