Ac-DEVD-AMC

• CAT Number: I013092
• CAS Number: 169332-61-0
• Molecular Formula: C₃₀H₃₇N₅O₁₃
• Molecular Weight: 675.64
• Purity: ≥95%
• Target: PeptidesDye Reagents
• Solubility: DMSO
• IUPAC Name: (4S)-4-[[(2S)-2-acetamido-3-carboxypropanoyl]amino]-5-[[(2S)-1-[[(2S)-3-carboxy-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopropan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-5-oxopentanoic acid
• InChI: InChI=1S/C30H37N5O13/c1-13(2)26(35-27(44)18(7-8-22(37)38)33-29(46)19(11-23(39)40)31-15(4)36)30(47)34-20(12-24(41)42)28(45)32-16-5-6-17-14(3)9-25(43)48-21(17)10-16/h5-6,9-10,13,18-20,26H,7-8,11-12H2,1-4H3,(H,31,36)(H,32,45)(H,33,46)(H,34,47)(H,35,44)(H,37,38)(H,39,40)(H,41,42)/t18-,19-,20-,26-/m0/s1
• InChIKey: ALZSTTDFHZHSCA-RNVDEAKXSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)C

Ac-DEVD-AMC（Cat No.:I013092）is a synthetic peptide substrate used in assays to measure caspase-3 activity, a key enzyme in the execution phase of apoptosis (programmed cell death). The peptide sequence “DEVD” is specifically recognized and cleaved by caspase-3. The “AMC” (7-amino-4-methylcoumarin) group is a fluorescent marker, which emits fluorescence upon cleavage of the peptide. This allows for quantitative detection of caspase-3 activation. Ac-DEVD-AMC is widely used in apoptosis research, drug discovery, and screening to assess the role of caspase-3 in cellular responses to various stimuli and potential therapeutic interventions.

Reference

[1]. Ahmed R Alsuwaidi, et al. Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol J. 2013; 10: 22. [2]. Ahmed R Alsuwaidi, et al. Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes. 2013; 6: 12.