AC-green

• CAT Number: I045000
• CAS Number: 2937705-58-1
• Molecular Formula: C24H25N3O5
• Molecular Weight: 435.47
• Purity: ≥95%
• Synonyms: 2-[[7-(diethylamino)-2-oxochromen-3-yl]carbamoyl]prop-2-enyl N-phenylcarbamate
• InChI: InChI=1S/C24H25N3O5/c1-4-27(5-2)19-12-11-17-13-20(23(29)32-21(17)14-19)26-22(28)16(3)15-31-24(30)25-18-9-7-6-8-10-18/h6-14H,3-5,15H2,1-2H3,(H,25,30)(H,26,28)
• InChIKey: PHBVJYLDYIFURJ-UHFFFAOYSA-N
• SMILES: CCN(CC)C1=CC2=C(C=C1)C=C(C(=O)O2)NC(=O)C(=C)COC(=O)NC3=CC=CC=C3

AC-green (VDP-green) is a β-allyl carbamate fluorescent probe for specifically imaging vicinal dithiol proteins (VDPs) in living systems (λex/λem=400/475 nm). AC-green can detect the reduced bovine serum albumin (rBSA) with high sensitivity. AC-green displays low toxicity and features high sensitivity, and is suitable for sensing VDPs in living cells and zebrafishes[1].
AC-green (VDP-green) can respond to VDPs with more than 60-fold increase of emission in aqueous solution, while there is no significant interference from biological thiols, amino acids or inorganic salts[1].
AC-green (2 μM; 90 min) has no apparent fluorescence signal within the pH range of 5.0-9.0. Addition of rBSA turns on the fluorescence[1].
AC-gree (10 μM) has low cytotoxicity in HepG2 cells and Hela cells[1].
AC-gree (10 μM; for 15 min) images VDPs in living HepG2 cells and bright green fluorescence appeared. This fluorescence is inhibited when the cells are pretreated with PAO, a popular specificligand for protein vicinal dithiols[1].
AC-gree (10 μM; for 20 min) incubates with zebrafishes has a strong fluorescence signal appeared in the green channel[1].

Reference

[1]. Lanning Zhao, et al. A β-allyl carbamate fluorescent probe for vicinal dithiol proteins. Chem Commun (Camb). 2020 Mar 5;56(19):2857-2860.
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