Ac-IETD-AFC

For research use only. Not for therapeutic Use.

  • CAT Number: R065567
  • CAS Number: 211990-57-7
  • Molecular Formula: C31H38F3N5O12
  • Molecular Weight: 729.70
  • Purity: ≥95%
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Ac-IETD-AFC(Cat No.:R065567)is a synthetic peptide substrate used in biochemical assays to measure caspase-8 activity, a key enzyme in the extrinsic apoptosis pathway. The peptide sequence “IETD” is specifically recognized and cleaved by caspase-8. The “AFC” (7-amino-4-trifluoromethylcoumarin) group is a fluorogenic marker that emits fluorescence upon cleavage of the peptide. This allows for quantitative detection of caspase-8 activation. Ac-IETD-AFC is commonly used in apoptosis research, particularly in studies exploring the extrinsic apoptosis pathway, and to screen for potential compounds that modulate caspase-8 activity in therapeutic contexts.


Catalog Number R065567
CAS Number 211990-57-7
Synonyms

N-Acetyl-Ile-Glu-Thr-Asp-7-amino-4-Trifluoromethylcoumarin

Molecular Formula C31H38F3N5O12
Purity ≥95%
Target Caspase
Storage -20°C
IUPAC Name (4S)-4-[[(2S,3S)-2-acetamido-3-methylpentanoyl]amino]-5-[[(2S,3R)-1-[[(2S)-3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-5-oxopentanoic acid
InChI InChI=1S/C31H38F3N5O12/c1-5-13(2)25(35-15(4)41)29(49)37-19(8-9-22(42)43)27(47)39-26(14(3)40)30(50)38-20(12-23(44)45)28(48)36-16-6-7-17-18(31(32,33)34)11-24(46)51-21(17)10-16/h6-7,10-11,13-14,19-20,25-26,40H,5,8-9,12H2,1-4H3,(H,35,41)(H,36,48)(H,37,49)(H,38,50)(H,39,47)(H,42,43)(H,44,45)/t13-,14+,19-,20-,25-,26-/m0/s1
InChIKey CGUOWXRXLQXOKO-FCQBACSVSA-N
SMILES CC[C@H](C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C
Reference

1.Reszka, P.,Schulz, R.,Methling, K., et al. Synthesis, enzymatic evaluation, and docking studies of fluorogenic caspase 8 tetrapeptide substrates. ChemMedChem 5, 103-117 (2010).

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