Ac-LEHD-AFC

• CAT Number: R024438
• CAS Number: 210345-03-2
• Molecular Formula: C33H38F3N7O11
• Molecular Weight: 765.70
• Purity: ≥95%
• Synonyms: N-Acetyl-L-leucyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-Asparagine
• Target: Caspase
• Solubility: Soluble to 1 mg/ml in sterile water
• Storage: Desiccate at -20°C
• IUPAC Name: (4S)-4-[[(2S)-2-acetamido-4-methylpentanoyl]amino]-5-[[(2S)-1-[[(2S)-3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-5-oxopentanoic acid
• InChI: InChI=1S/C33H38F3N7O11/c1-15(2)8-22(39-16(3)44)31(52)41-21(6-7-26(45)46)29(50)42-23(9-18-13-37-14-38-18)32(53)43-24(12-27(47)48)30(51)40-17-4-5-19-20(33(34,35)36)11-28(49)54-25(19)10-17/h4-5,10-11,13-15,21-24H,6-9,12H2,1-3H3,(H,37,38)(H,39,44)(H,40,51)(H,41,52)(H,42,50)(H,43,53)(H,45,46)(H,47,48)/t21-,22-,23-,24-/m0/s1
• InChIKey: HULKIXRFKRCRHD-ZJZGAYNASA-N
• SMILES: CC(C)C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC1=CN=CN1)C(=O)N[C@@H](CC(=O)O)C(=O)NC2=CC3=C(C=C2)C(=CC(=O)O3)C(F)(F)F)NC(=O)C

Ac-LEHD-AFC（Cat No.:R024438）is a synthetic peptide substrate used in biochemical assays to measure caspase-9 activity. The peptide consists of the sequence LEHD (which is a recognition site for caspase-9) conjugated to a fluorescent molecule, AFC (7-amino-4-trifluoromethylcoumarin). When cleaved by caspase-9, the peptide releases the AFC moiety, which emits fluorescence that can be quantitatively measured. This assay is commonly used in cell biology and apoptosis research to study caspase-9 activation, which is a critical enzyme in the intrinsic pathway of apoptosis (programmed cell death), allowing for the evaluation of cellular responses to various stimuli.