For research use only. Not for therapeutic Use.
Ac-PAL-AMC（Cat No.:I042957）is a fluorogenic peptide substrate commonly used in enzyme activity assays, particularly for studying proteases such as serine proteases. The substrate consists of an acetyl group (Ac) attached to the N-terminus of a peptide sequence (PAL), which is linked to the fluorescent reporter molecule 7-amino-4-methylcoumarin (AMC). When the enzyme cleaves the peptide bond, AMC is released, producing fluorescence that can be measured to quantify enzyme activity. Ac-PAL-AMC is widely utilized in biochemical research for screening protease inhibitors and studying enzyme kinetics, providing insights into protease function and regulation.
| CAS Number | 1431362-79-6 |
| Synonyms | (2S)-1-acetyl-N-[(2S)-1-[[(2S)-4-methyl-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]pyrrolidine-2-carboxamide |
| Molecular Formula | C26H34N4O6 |
| Purity | ≥95% |
| IUPAC Name | (2S)-1-acetyl-N-[(2S)-1-[[(2S)-4-methyl-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]pyrrolidine-2-carboxamide |
| InChI | InChI=1S/C26H34N4O6/c1-14(2)11-20(25(34)28-18-8-9-19-15(3)12-23(32)36-22(19)13-18)29-24(33)16(4)27-26(35)21-7-6-10-30(21)17(5)31/h8-9,12-14,16,20-21H,6-7,10-11H2,1-5H3,(H,27,35)(H,28,34)(H,29,33)/t16-,20-,21-/m0/s1 |
| InChIKey | BWJPVHDZSJFFDM-NDXORKPFSA-N |
| SMILES | CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]3CCCN3C(=O)C |
