Ac-VDVAD-AFC

• CAT Number: R064942
• CAS Number: 210344-94-8
• Molecular Formula: C33H41F3N6O12
• Molecular Weight: 770.72
• Purity: ≥95%
• Synonyms: N-Acetyl-Val-Asp-Val-Ala-Asp-7-amido-4-Trifluoromethylcoumarin;Caspase-2 Substrate (Fluorogenic)
• Storage: -20°C
• IUPAC Name: 3-[2-[[2-[[2-[(2-acetamido-3-methylbutanoyl)amino]-3-carboxypropanoyl]amino]-3-methylbutanoyl]amino]propanoylamino]-4-oxo-4-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]butanoic acid
• InChI: InChI=1S/C33H41F3N6O12/c1-13(2)26(38-16(6)43)32(53)41-21(12-24(46)47)30(51)42-27(14(3)4)31(52)37-15(5)28(49)40-20(11-23(44)45)29(50)39-17-7-8-18-19(33(34,35)36)10-25(48)54-22(18)9-17/h7-10,13-15,20-21,26-27H,11-12H2,1-6H3,(H,37,52)(H,38,43)(H,39,50)(H,40,49)(H,41,53)(H,42,51)(H,44,45)(H,46,47)
• InChIKey: BKQMJQIAIFEZJR-UHFFFAOYSA-N
• SMILES: CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC(C(C)C)C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C

Ac-VDVAD-AFC(CAT: R064942) is a synthetic peptide substrate used in biochemical research to detect caspase-2 activity. It consists of the amino acid sequence VDVAD, which is specifically recognized and cleaved by caspase-2, an important enzyme involved in apoptosis, or programmed cell death. The cleavage of this substrate releases AFC (7-amino-4-trifluoromethylcoumarin), a fluorescent molecule, allowing researchers to measure caspase-2 activity through fluorescence detection. This makes Ac-VDVAD-AFC a valuable tool in studies focused on apoptosis, cancer research, and drug discovery, where monitoring caspase activation is critical for understanding cellular death pathways.