For research use only. Not for therapeutic Use.
Ac-YVAD-AFC (Cat No.:R029939) is a fluorogenic peptide substrate widely used in caspase-1 activity assays, apoptosis research, and drug discovery. The YVAD sequence is highly specific for caspase-1 (ICE, interleukin-1β converting enzyme), making it valuable for studying inflammatory pathways and cell death mechanisms. Upon enzymatic cleavage, the AFC (7-amino-4-trifluoromethylcoumarin) fluorophore is released, producing a measurable fluorescence signal. This compound is essential in biochemical assays, inhibitor screening, and therapeutic research, particularly for developing caspase-targeted drugs and understanding immune responses in inflammation and disease models.
| CAS Number | 219137-85-6 |
| Synonyms | (3S)-3-[[(2S)-2-[[(2S)-2-[[(2S)-2-acetamido-3-(4-hydroxyphenyl)propanoyl]amino]-3-methylbutanoyl]amino]propanoyl]amino]-4-oxo-4-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]butanoic acid |
| Molecular Formula | C33H36F3N5O10 |
| Purity | ≥95% |
| IUPAC Name | (3S)-3-[[(2S)-2-[[(2S)-2-[[(2S)-2-acetamido-3-(4-hydroxyphenyl)propanoyl]amino]-3-methylbutanoyl]amino]propanoyl]amino]-4-oxo-4-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]butanoic acid |
| InChI | InChI=1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)/t16-,23-,24-,28-/m0/s1 |
| InChIKey | GWKJISJKNKIJNP-DEQDFGQZSA-N |
| SMILES | C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC3=CC=C(C=C3)O)NC(=O)C |
| Chemistry Calculators | Dilution Calculator In vivo Formulation Calculator Molarity Calculator Molecular Weight Calculator Reconstitution Calculator |
