Acridine Orange 10-Nonyl Bromide

• CAT Number: I013032
• CAS Number: 75168-11-5
• Molecular Formula: C₂₆H₃₈BrN₃
• Molecular Weight: 472.50
• Purity: ≥95%
• Target: Dye Reagents
• Solubility: DMSO
• IUPAC Name: 3-N,3-N,6-N,6-N-tetramethyl-10-nonylacridin-10-ium-3,6-diamine;bromide
• InChI: InChI=1S/C26H38N3.BrH/c1-6-7-8-9-10-11-12-17-29-25-19-23(27(2)3)15-13-21(25)18-22-14-16-24(28(4)5)20-26(22)29;/h13-16,18-20H,6-12,17H2,1-5H3;1H/q+1;/p-1
• InChIKey: DRBHTUDHPPBMCD-UHFFFAOYSA-M
• SMILES: CCCCCCCCC[N+]1=C2C=C(C=CC2=CC3=C1C=C(C=C3)N(C)C)N(C)C.[Br-]

Acridine Orange 10-Nonyl Bromide (Cat No.:I013032) is a fluorescent compound used in biological research, particularly for the study of cell membrane dynamics and endocytosis. It is a derivative of Acridine Orange, a well-known dye that binds to nucleic acids and exhibits fluorescence. AONB includes a nonyl group, enhancing its lipophilicity, allowing it to penetrate cell membranes more efficiently. The compound can be used to track cellular processes, such as membrane trafficking, and to assess the integrity of the plasma membrane. Its fluorescence properties make it useful in imaging studies of living cells.

Reference

[1]. Ratinaud MH, et al. In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes. Cytometry. 1988 May;9(3):206-12.[2]. Gallet PF, et al. Direct cardiolipin assay in yeast using the red fluorescence emission of 10-N-nonyl acridine orange. Eur J Biochem. 1995 Feb 15;228(1):113-9.