Acridine Orange

• CAT Number: R064768
• CAS Number: 494-38-2
• Molecular Formula: C17H19N3
• Molecular Weight: 265.36
• Purity: ≥95%
• Synonyms: C.I. 46005B;NSC 194350
• Storage: Room temperature
• IUPAC Name: 3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine
• InChI: InChI=1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3
• InChIKey: DPKHZNPWBDQZCN-UHFFFAOYSA-N
• SMILES: CN(C)C1=CC2=C(C=C1)C=C3C=CC(=CC3=N2)N(C)C

Acridine orange is a cell-permeable, nucleic acid-selective fluorescent cationic dye useful for cell cycle determination. It emits green fluorescence (excitation maximum: 502 nm; emission maximum: 525 nm) when bound to dsDNA and red fluorescence (excitation maximum: 460 nm; emission maximum: 650 nm) when bound to ssDNA or RNA. Acridine orange will also enter acidic compartments such as lysosomes and become protonated and sequestered. In low pH conditions such as this, acridine orange will emit orange fluorescence (emission ~590 nm) when excited by blue light (475 nm).