Autophagy inducer 3

• CAT Number: I043692
• CAS Number: 2691054-63-2
• Molecular Formula: C24H43NO2
• Molecular Weight: 377.60
• Purity: ≥95%
• Synonyms: (2S,3S)-2-amino-1-(4-methoxyphenyl)heptadecan-3-ol
• InChI: InChI=1S/C24H43NO2/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-24(26)23(25)20-21-16-18-22(27-2)19-17-21/h16-19,23-24,26H,3-15,20,25H2,1-2H3/t23-,24-/m0/s1
• InChIKey: HMKPXKFKBCPUEK-ZEQRLZLVSA-N
• SMILES: CCCCCCCCCCCCCCC(C(CC1=CC=C(C=C1)OC)N)O

Autophagy inducer 3 has autophagy induced activity. Autophagy inducer 3 possesses robust autophagic cell death in diverse cancer cells sparing normal counterpart. Autophagy inducer 3 induces lethal autophagy by formation of characteristic autophagic vacuoles, LC3 puncta formation, upregulation of signature autophagy markers like Beclin and Atg family proteins[1].
Autophagy inducer 3 (compound 26b) (10 μM; 48 hours) effectively inhibits cell growth in diverse breast, lung and colon cancer cell lines[1].
Autophagy inducer 3 (0-10 μM; 48 hours) exhibits potent antiproliferative activity in COLO-205, LOVO, HT-29, DLD-1, SW48 and SW-620 with IC50s of 2.03 μM, 3.33 μM, 4.15 μM, 4.46 μM, 3.14 μM, 1.86 μM, respectively; and shows low cytotoxicity in normal human colon fibroblast CCD-18Co with IC50 over 10 μM[1].
Autophagy inducer 3 (7.5 μM; 18 hours) promotes non apoptotic cell death in DLD-1 cells by cellular granularity through vacuole formation and increase Annexin-V positive cells as well as PARP cleavage; and robustly induces the expression of classical autophagy markers like Beclin-1, Atg3, Atg5 and Atg7[1].

Reference

[1]. Ganesher A, et al. New Spisulosine Derivative promotes robust autophagic response to cancer cells. Eur J Med Chem. 2020;188:112011.
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