For research use only. Not for therapeutic Use.
AY 9944 is a specific cholesterol biosynthesis inhibitor. AY 9944 inhibits the 7-dehydro cholesterol Δ7-reductase (DHCR7) enzyme with an IC50 of 13 nM. AY 9944 causes hypocholesterolemia and accumulation of 7DHC. At high doses, AY 9944 inhibits also in cultured embryos sterol Δ7-Δ8 isomerase, which causes the accumulation of cholest-8-en-3β-ol[1][2][3].
AY 9944 (1 μg/mL; 15 h) affects types of free sterol in keratinocytes[2].
AY 9944 (7.5 mg/kg; i.h. every 6 days once from postnatal day 2 to postnatal day 20) reduces brain cholesterol until 400 days after stop treatment[3].
| Catalog Number | R009397 |
| CAS Number | 366-93-8 |
| Synonyms | N-[(2-chlorophenyl)methyl]-1-[4-[[(2-chlorophenyl)methylamino]methyl]cyclohexyl]methanamine;dihydrochloride |
| Molecular Formula | C22H30Cl4N2 |
| Purity | ≥95% |
| InChI | InChI=1S/C22H28Cl2N2.2ClH/c23-21-7-3-1-5-19(21)15-25-13-17-9-11-18(12-10-17)14-26-16-20-6-2-4-8-22(20)24;;/h1-8,17-18,25-26H,9-16H2;2*1H |
| InChIKey | NRVIEWRSGDDWHP-UHFFFAOYSA-N |
| SMILES | C1CC(CCC1CNCC2=CC=CC=C2Cl)CNCC3=CC=CC=C3Cl.Cl.Cl |
| Reference | [1]. Moebius FF, et al. Molecular cloning and expression of the human delta7-sterol reductase. Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1899-902. [2]. Nemanic MK, et al. In vitro synthesis of vitamin D-3 by cultured human keratinocytes and fibroblasts: action spectrum and effect of AY-9944. Biochim Biophys Acta. 1985 Sep 6;841(3):267-77. [3]. Cortez MA, et al. Brain sterols in the AY-9944 rat model of atypical absence seizures. Epilepsia. 2002 Jan;43(1):3-8. |
