Azidobutyric acid NHS ester

• CAT Number: I022906
• CAS Number: 943858-70-6
• Molecular Formula: C8H10N4O4
• Molecular Weight: 226.19
• Purity: 98%
• Target: ADC Linker
• Solubility: Soluble in DMSO
• Appearance: Solid powder
• Storage: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
• IUPAC Name: Butanoic acid, 4-​azido-​, 2,​5-​dioxo-​1-​pyrrolidinyl ester
• InChI: InChI=1S/C8H10N4O4/c9-11-10-5-1-2-8(15)16-12-6(13)3-4-7(12)14/h1-5H2
• InChIKey: YYULROINNKAMIB-UHFFFAOYSA-N
• SMILES: O=C(ON1C(CCC1=O)=O)CCCN=[N+]=[N-]

Azidobutyric acid NHS ester (Cat No.:I022906) is a heterobifunctional crosslinking reagent that contains an azide group and an N-hydroxysuccinimide (NHS) ester. It is commonly used in bioconjugation reactions to attach azide groups to primary amine groups of proteins, peptides, or other molecules. The azide group can then be reacted with other molecules containing a terminal alkyne group via a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, allowing for the specific labeling or modification of biological molecules.

Reference

1. Hou, W.; Li, Y.; Kang, W.; Wang, X.; Wu, X.; Wang, S.; Liu, F. Real-time analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the microtubules using single particle tracking. Scientific Reports, 2019, 9, 1307. doi: 10.1038/s41598-018-37789-9
2.Kuznetsov, A.E.; Komarova, N.V.; Kuznetsov, E.V.; Andrianova, M.S.; Grudtsov, V.P.; Rybachek, E.N.; Puchnin, K.V.; Ryazantsev, D.V.; Saurov, A.N. Integration of a field effect transistor-based aptasensor under a hydrophobic membrane for bioelectronic nose applications. Biosensors and Bioelectronics, 2019, 129, 29–35. doi: 10.1016/j.bios.2019.01.013
3.Kumar, P.; Kuhlmann, F.M.; Chakroborty, S.; Bourgeois, A.L.; Foulke-Abel, J.; Tumala, B.; Vickers, T.J.; Sack, D.A.; DeNearing, B.; Harro, C.D.; Wright, W.S.; Gildersleeve, J.C.; Ciorba, M.A.; Santhanam, S.; Porter, C.K.; Gutierrez, R.L.; Prouty, M.G.; Riddle, M.S.; Polino, A.; Sheikh, A.; Donowitz, M.; Fleckenstein, J.M. Enterotoxigenic Escherichia coli blood group A interactions intensify diarrheal severity. The Journal of Clinical Investigation, 2018, 128(8), 3298–3311. doi: 10.1172/JCI97659
4.Andrianova, M.; Komarova, N.; Grudtsov, V.; Kuznetsov, E.; Kuznetsov, A. Amplified Detection of the Aptamer-Vanillin Complex with the Use of Bsm DNA Polymerase. Sensors, 2018, 18, 49. doi: 10.3390/s18010049