For research use only. Not for therapeutic Use.
BCECF is a pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0–8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Prepare a 2-20 mM stock solution of BCECF in DMSO and store at -20℃.
2. Prepare a 5-50 μM BCECF dye-loading solution in buffer solutions (Hepes, pH 7.4 or PBS).
3. Add 1000 μL/well (6-well plate),100 μL/well (96-well plate) or 25 μL/well (384-well plate) BCECF dye-loading solution into the cell plate.
4. Incubate the dye-loading plate in a cell incubator about 30minutes.
5. Wash and replace the dye-loading solution with buffers.
6. Run the pH assay by monitoring the fluorescence at Ex/Em = 490/535 nm or 430/535 nm for ratio measurements.
| Catalog Number | I013223 |
| CAS Number | 85138-49-4 |
| Synonyms | 2′,7′-bis(2-carboxyethyl)-3′,6′-dihydroxy-1-oxospiro[2-benzofuran-3,9′-xanthene]-5-carboxylic acid;2′,7′-bis(2-carboxyethyl)-3′,6′-dihydroxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-carboxylic acid |
| Molecular Formula | C54H40O22 |
| Purity | ≥95% |
| InChI | InChI=1S/2C27H20O11/c28-19-10-21-17(8-12(19)2-5-23(30)31)27(16-4-1-14(25(34)35)7-15(16)26(36)38-27)18-9-13(3-6-24(32)33)20(29)11-22(18)37-21;28-19-10-21-17(7-12(19)2-5-23(30)31)27(16-9-14(25(34)35)1-4-15(16)26(36)38-27)18-8-13(3-6-24(32)33)20(29)11-22(18)37-21/h2*1,4,7-11,28-29H,2-3,5-6H2,(H,30,31)(H,32,33)(H,34,35) |
| InChIKey | RZFSVSQVNQXHLI-UHFFFAOYSA-N |
| SMILES | C1=CC2=C(C=C1C(=O)O)C3(C4=C(C=C(C(=C4)CCC(=O)O)O)OC5=C3C=C(C(=C5)O)CCC(=O)O)OC2=O.C1=CC2=C(C=C1C(=O)O)C(=O)OC23C4=C(C=C(C(=C4)CCC(=O)O)O)OC5=C3C=C(C(=C5)O)CCC(=O)O |
| Reference | [1]. Scott DA, et al. Analysis of the uptake of the fluorescent marker 2′,7′-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) by hydrogenosomes in Trichomonas vaginalis. Eur J Cell Biol. 1998 Jun;76(2):139-45. |
