BDP 558/568 NHS ester

• CAT Number: I043056
• CAS Number: 150173-73-2
• Molecular Formula: C20H16BF2N3O4S
• Molecular Weight: 443.23
• Purity: ≥95%

BDP 558/568 NHS ester is a borondipyrromethene fluorophore with emission in the yellow part of the spectrum. BDP 558/568 NHS ester is an amine reactive NHS ester, and the absorption and emission spectra of BDP 558/568 NHS ester are similar with TAMRA, BDP TMR, Cyanine3, and sulfo-Cyanine3[1][2].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Suspend sample in 0.5 M sodium bicarbonate, pH 9.0, and incubates with BDP 558/568 NHS ester for 30 min at a room temperature with frequent sonication.
2. Incubate BODIPY-sample complex with 1 mg/mL FBS for 10 min at 37℃.
3. Investigate fluorescence of BODIPY-sample complex (Ex=558 nm, Em=568 nm)[1][2].

Reference

[1]. Vasselon T, et al. Internalization of monomeric lipopolysaccharide occurs after transfer out of cell surface CD14. J Exp Med. 1999 Aug 16;190(4):509-21.
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[2]. Yu B, Wright SD. Catalytic properties of lipopolysaccharide (LPS) binding protein. Transfer of LPS to soluble CD14. J Biol Chem. 1996 Feb 23;271(8):4100-5.
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