BES

• CAT Number: M122209
• CAS Number: 10191-18-1
• Molecular Formula: C6H15NO5S
• Molecular Weight: 213.248
• Purity: ≥95%
• Storage: -20°C
• IUPAC Name: 2-[bis(2-hydroxyethyl)amino]ethanesulfonic acid
• InChI: InChI=1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
• InChIKey: AJTVSSFTXWNIRG-UHFFFAOYSA-N
• SMILES: C(CO)N(CCO)CCS(=O)(=O)O

BES (CAS 10191-18-1) buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. It is used as an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.

Reference

1.De Graeve, K. G., et al. "Competition between reductive acetogenesis and methanogenesis in the pig large‐intestinal flora." Journal of Applied Bacteriology 76.1 (1994): 55-61.
2.Chen, Claudia A., and Hiroto Okayama. "Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA." Biotechniques 6.7 (1988): 632-638.