Beta-Sitosterol

• CAT Number: I005010
• CAS Number: 83-46-5
• Molecular Formula: C29H50O
• Molecular Weight: 414.7
• Purity: ≥95%
• Synonyms: Azuprostat;Betaprost;Cupreol;NSC 18173;NSC 49083;NSC 8096;Rhamnol;β-Sitosterol;SKF 14463;22,23-dihydro-Stigmasterol
• Target: Apoptosis
• Solubility: DMSO
• Storage: 3 years -20C powder
• InChIKey: KZJWDPNRJALLNS-VJSFXXLFSA-N

Beta-Sitosterol weakly inhibits porcine pancreatic lipase (PPL) activity. Sitosterol is an important compound extracted from the leaves of Aloe vera.IC50 & Target: 99.99±1.86 μg/mL (PPL)[1]InVitro: Bioactivity-guided isolation afforded three compounds from the hexane fraction of E. indica, namely, β-sitosterol, stigmasterol, and lutein. Both compounds are found to possess very low PPL inhibition activity, that is, 2.99±0.80% (β-sitosterol) of inhibition at 100 μg/mL (242 μM) and 2.68±0.38% (stigmasterol) of inhibition at 100 μg/mL (243 μM), respectively. Weak PPL inhibition activity of β-sitosterol and stigmasterol isolated from Alpinia zerumbet with IC50 value of 99.99±1.86 μg/mL and 125.05±4.76 μg/mL, respectively, in comparison with the inhibition shown by curcumin (IC50=4.92±0.21 μg/mL) and quercetin (IC50=18.60±0.86 μg/mL) which are used as positive controls in their study. β-sitosterol and stigmasterol are recorded with weak PPL inhibitory activity of only 3.0±0.8% and 2.7±0.4% at 100 μg/mL, respectively, (i.e., 242 μM and 243 μM) in contrast (34.5±5.4% at 100 μg/mL), which are comparatively lower than that recorded in literature (i.e., 50% PPL inhibition at 100 μg/mL)[1]. Sitosterol is an important compound extracted from the leaves of Aloe vera. It inhibits the growth of promastigotes of L. donovani, a causative agent for life threatening visceral leishmaniasis disease[2].InVivo: β-sitosterol treatment significantly reduced the immobility time at three doses (10, 20, and 30 mg/kg) in the Forced Swim Test (FST) and Tail Suspension Test (TST), indicating an antidepressant effect. This effect is similar to the positive control fluoxetine (20 mg/kg) at a dose of 30 mg/kg, where the strongest effect is observed compared with the control group (P < 0.001). The same effects are observed for three doses of β-sitosterol in the TST. The % DID values are as follows: FST: 39.27% (10 mg/kg), 51.23% (20 mg/kg), and 57.48% (30 mg/kg); TST: 31.63% (10 mg/kg), 43.95% (20 mg/kg), and 53.38% (30 mg/kg). These results indicate that β-sitosterol has a significant antidepressant activity in mice during the FST and TST. Furthermore, β-sitosterol exhibits the antidepressant effect in a dose-dependent manner[3].

Reference

[1]. Ong SL, et al. Porcine Pancreatic Lipase Inhibitory Agent Isolated from Medicinal Herb and Inhibition Kinetics of Extracts from Eleusine indica (L.) Gaertner. J Pharm (Cairo). 2016;2016:8764274.

[2]. Tariq A, et al. Ethnomedicines and anti-parasitic activities of Pakistani medicinal plants against Plasmodia and Leishmania parasites. Ann Clin Microbiol Antimicrob. 2016 Sep 20;15(1):52.

[3]. Zhao D, et al. Structural Features and Potent Antidepressant Effects of Total Sterols and β-sitosterol Extracted from Sargassum horneri. Mar Drugs. 2016 Jun 28;14(7).