BHQ-2

• CAT Number: I012779
• CAS Number: 1214891-99-2
• Molecular Formula: C25H26N6O6
• Molecular Weight: 506.519
• Purity: ≥95%
• Synonyms: BHQ-2 Quencher; Black Hole Quencher-2; 4-[[4-[2-[2,5-Dimethoxy-4-[2-(4-nitrophenyl)diazenyl] phenyl]diazenyl]phenyl]methylamino]-butanoic acid; BHQ 2 Carboxylic Acid; Black Hole Quencher 2 Carboxyl
• IUPAC Name: 4-[4-[[2,5-dimethoxy-4-[(4-nitrophenyl)diazenyl]phenyl]diazenyl]-N-methylanilino]butanoic acid
• InChI: InChI=1S/C25H26N6O6/c1-30(14-4-5-25(32)33)19-10-6-17(7-11-19)26-28-21-15-24(37-3)22(16-23(21)36-2)29-27-18-8-12-20(13-9-18)31(34)35/h6-13,15-16H,4-5,14H2,1-3H3,(H,32,33)
• InChIKey: UDGUGZTYGWUUSG-UHFFFAOYSA-N
• SMILES: CN(CCCC(=O)O)C1=CC=C(C=C1)N=NC2=CC(=C(C=C2OC)N=NC3=CC=C(C=C3)[N+](=O)[O-])OC

BHQ-2(CAT: I012779) is a compound known as a Black Hole Quencher-2. It is a fluorescence quencher commonly used in molecular biology and diagnostics. BHQ-2 is designed to efficiently quench the fluorescence signal emitted by nearby fluorophores. It is often used as a non-fluorescent reporter molecule in various applications, including real-time polymerase chain reaction (PCR) assays, nucleic acid hybridization probes, and fluorescence resonance energy transfer (FRET) assays. BHQ-2 acts by absorbing and dissipating the energy from the fluorophore, resulting in the suppression of fluorescence emission. This quenching property makes BHQ-2 useful for fluorescence-based detection and analysis in molecular biology research and diagnostics.