Biotin-PEG3-NHS ester (CAT: I014201) is a bioconjugation reagent widely used in various applications involving the modification of biomolecules. This compound contains a biotin group, a polyethylene glycol (PEG) spacer of three units, and an N-hydroxysuccinimide (NHS) ester functionality. The NHS ester moiety enables efficient and stable coupling of the reagent to primary amines on target molecules, such as proteins and peptides. Biotin-PEG3-NHS ester serves as a valuable tool in the development of bioconjugates, affinity-based assays, and studies involving the immobilization, labeling, or purification of biomolecules.
| Catalog Number | I014201 |
| CAS Number | 1253286-56-4 |
| Synonyms | Biotin-PEG3-NHS ester;2,5-dioxopyrrolidin-1-yl 14-oxo-18-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-4,7,10-trioxa-13-azaoctadecanoate |
| Molecular Formula | C23H36N4O9S |
| Purity | ≥95% |
| Solubility | Soluble in DMSO |
| InChI | InChI=1S/C23H36N4O9S/c28-18(4-2-1-3-17-22-16(15-37-17)25-23(32)26-22)24-8-10-34-12-14-35-13-11-33-9-7-21(31)36-27-19(29)5-6-20(27)30/h16-17,22H,1-15H2,(H,24,28)(H2,25,26,32)/t16-,17-,22-/m0/s1 |
| InChIKey | QIAUJZJSFIYGMH-HOIFWPIMSA-N |
| SMILES | O=C1N[C@@]2([H])CS[C@@H](CCCCC(NCCOCCOCCOCCC(ON3C(CCC3=O)=O)=O)=O)[C@@]2([H])N1 |
| Reference | </br>1: Deng CY, Long YY, Liu S, Chen ZB, Li C. [Construction of biotin-modified polymeric micelles for pancreatic cancer targeted photodynamic therapy]. Yao Xue Xue Bao. 2015 Aug;50(8):1038-44. Chinese. PubMed PMID: 26669006.</br>2: Zhu W, Song Z, Wei P, Meng N, Teng F, Yang F, Liu N, Feng R. Y-shaped biotin-conjugated poly (ethylene glycol)-poly (epsilon-caprolactone) copolymer for the targeted delivery of curcumin. J Colloid Interface Sci. 2015 Apr 1;443:1-7. doi: 10.1016/j.jcis.2014.11.073. Epub 2014 Dec 6. PubMed PMID: 25526295.</br>3: Ke S, Wright JC, Kwon GS. Avidin-biotin-PEG-CPA complexes as potential EPR-directed therapeutic protein carriers: preparation and characterization. Bioconjug Chem. 2007 Sep-Oct;18(5):1644-50. Epub 2007 Aug 18. PubMed PMID: 17705554.</br>4: Kaiser K, Marek M, Haselgrübler T, Schindler H, Gruber HJ. Basic studies on heterobifunctional biotin-PEG conjugates with a 3-(4-pyridyldithio)propionyl marker on the second terminus. Bioconjug Chem. 1997 Jul-Aug;8(4):545-51. PubMed PMID: 9258454. </br> </br> |
