Bis-ANS

• CAT Number: I011330
• CAS Number: 65664-81-5
• Molecular Formula: C32H22K2N2O6S2
• Molecular Weight: 672.85
• Purity: ≥95%
• Synonyms: 4,4/’-Bis(phenylamino)-[1,1/’-binaphthalene]-5,5/’-disulfonic acid dipotassium salt
• Target: Cytoskeleton
• Solubility: Soluble to 100 mM in DMSO and to 20 mM in water
• Storage: Store at RT
• IUPAC Name: dipotassium;8-anilino-5-(4-anilino-5-sulfonatonaphthalen-1-yl)naphthalene-1-sulfonate
• InChI: InChI=1S/C32H24N2O6S2.2K/c35-41(36,37)29-15-7-13-25-23(17-19-27(31(25)29)33-21-9-3-1-4-10-21)24-18-20-28(34-22-11-5-2-6-12-22)32-26(24)14-8-16-30(32)42(38,39)40;;/h1-20,33-34H,(H,35,36,37)(H,38,39,40);;/q;2*+1/p-2
• InChIKey: VWLWTJHKQHRTNC-UHFFFAOYSA-L
• SMILES: C1=CC=C(C=C1)NC2=C3C(=C(C=C2)C4=C5C=CC=C(C5=C(C=C4)NC6=CC=CC=C6)S(=O)(=O)[O-])C=CC=C3S(=O)(=O)[O-].[K+].[K+]

Bis-ANS (Cat.No:I011330), also known as 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid, is a fluorescent dye used to study protein folding, binding, and aggregation. It is highly sensitive to hydrophobic environments and has been used to detect protein unfolding and misfolding. Bis-ANS binds to exposed hydrophobic regions in proteins, resulting in a shift in its fluorescence spectra. It has also been used to study the binding of small molecules to proteins and the interaction of proteins with membranes.