Blasticidin S Hydrochloride

• CAT Number: R024204
• CAS Number: 3513-03-9
• PubChem Substance ID: 25078183
• Molecular Formula: C₁₇H₂₆N₈O₅ · HCl
• Molecular Weight: 458.9
• Purity: ≥95%
• Synonyms: Blasticidin S Monohydrochloride; (S)-4-[[3-Amino-5-[(aminoiminomethyl)methylamino]-1-oxopentyl]amino]-1-(4-amino-2-oxo-1(2H)-pyrimidinyl)-1,2,3,4-tetradeoxy-β-D-erythro-Hex-2-enopyranuronic Acid Monohydrochloride
• Target: DNA Synthesis
• Solubility: Limited solubility
• Appearance: White solid
• Storage: Store at -20°C
• IUPAC Name: (2S,3S,6R)-3-[[(3R)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylic acid;hydrochloride
• InChI: InChI=1S/C17H26N8O5.ClH/c1-24(16(20)21)6-4-9(18)8-12(26)22-10-2-3-13(30-14(10)15(27)28)25-7-5-11(19)23-17(25)29;/h2-3,5,7,9-10,13-14H,4,6,8,18H2,1H3,(H3,20,21)(H,22,26)(H,27,28)(H2,19,23,29);1H/t9-,10+,13-,14+;/m1./s1
• InChIKey: YQXYQOXRCNEATG-ZAYJLJTISA-N
• SMILES: CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl

Blasticidin S HCl is a nucleoside antibiotic derived from Streptomyces griseochromogenes. Blasticidin S HCl is routinely used in cell culture gene selection applications as a selectable agent to isolate blasticidin S resistant mammalian and bacterial cells.

Reference

Yamaguchi, I., et al. /Expression of the Blasticidin S Deaminase Gene (bsr) in Tobacco: Fungicide Tolerance and a New Selective Marker for Transgenic Plants./ Molecular and General Genetics 223.2 (1990): 332-34. www.ncbi.gov. Web. 7 Sept. 2012.

/Blasticidin S Inhibits Translation by Trapping Deformed TRNA on the Ribosome./ PNAS 110.30 (2013): 12283-2288. Ncbi.nlm.nih.gov. Web. 4 Sept. 2014.

Bento, Fernanda M. /Over Expression of the Selectable Marker Blasticidin S Deaminase Gene Is Toxic to Human Keratinocytes and Murine BALB/MK Cells./ BMC Biotechnology 4.29 (2004): 1-10. Springer.com. BioMed Central. Web. 4 Nov. 2014.

Kimura, M. /Blasticidin S Deaminase Gene from Aspergillus Terreus (BSD): A New Drug Resistance Gene for Transfection of Mammalian Cells./ Biochemica Et Biophysica Acta. 1219.3 (1994): 653-59. Ncbi.nlm.nih.gov. Web. 4 Nov. 2014.