(-)-Blebbistatin

• CAT Number: I005143
• CAS Number: 856925-71-8
• Molecular Formula: C₁₈H₁₆N₂O₂
• Molecular Weight: 292.34
• Purity: ≥95%
• Synonyms: (S)-3a-hydroxy-6-methyl-1-phenyl-3,3a-dihydro-1H-pyrrolo[2,3-b]quinolin-4(2H)-one
• Target: Myosin
• Solubility: DMSO: ≥ 31 mg/mL
• Storage: Store at -20°C
• IC50: 0.5 - 5 μM
• InChI: InChI=1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3/t18-/m1/s1
• InChIKey: LZAXPYOBKSJSEX-GOSISDBHSA-N
• SMILES: CC1=CC2=C(C=C1)N=C3C(C2=O)(CCN3C4=CC=CC=C4)O

(-)-Blebbistatin (CAT: I005143) is a small molecule inhibitor commonly used in cell biology and pharmacological research. It is known for its selective inhibition of non-muscle myosin II ATPase activity, leading to the disruption of actin-myosin interactions. (-)-Blebbistatin has been widely utilized to study cellular processes that rely on actomyosin contractilities, such as cell motility, cytokinesis, and muscle contraction. It has also been used in various experimental models to investigate the role of non-muscle myosin II in developmental processes, cell migration, and tissue morphogenesis. (-)-Blebbistatin is a valuable tool compound for understanding the mechanisms underlying actomyosin dynamics and has potential applications in the development of therapeutic strategies targeting diseases involving aberrant myosin function.

Reference

1: Fedorov VV, Lozinsky IT, Sosunov EA, Anyukhovsky EP, Rosen MR, Balke CW,
Efimov IR. Application of blebbistatin as an excitation-contraction uncoupler for
electrophysiologic study of rat and rabbit hearts. Heart Rhythm. 2007
May;4(5):619-26. Epub 2007 Jan 7. PubMed PMID: 17467631.

2: Katayama T, Yoshiyama S, Tanaka H, Wang HH, Nakamura A, Kohama K. Blebbistatin
inhibits sphingosylphosphorylcholine-induced contraction of collagen-gel fiber
populated by vascular smooth-muscle cells. J Pharmacol Sci. 2006
Nov;102(3):339-42. Epub 2006 Oct 28. PubMed PMID: 17072099.

3: Shu S, Liu X, Korn ED. Blebbistatin and blebbistatin-inactivated myosin II
inhibit myosin II-independent processes in Dictyostelium. Proc Natl Acad Sci U S
A. 2005 Feb 1;102(5):1472-7. Epub 2005 Jan 25. PubMed PMID: 15671182; PubMed
Central PMCID: PMC547870.

4: Kolega J. Phototoxicity and photoinactivation of blebbistatin in UV and
visible light. Biochem Biophys Res Commun. 2004 Jul 30;320(3):1020-5. PubMed
PMID: 15240150.

5: Duxbury MS, Ashley SW, Whang EE. Inhibition of pancreatic adenocarcinoma
cellular invasiveness by blebbistatin: a novel myosin II inhibitor. Biochem
Biophys Res Commun. 2004 Jan 23;313(4):992-7. PubMed PMID: 14706640.