BODIPY 581/591 C11

• CAT Number: I023906
• CAS Number: 217075-36-0
• Molecular Formula: C30H35BF2N2O2
• Molecular Weight: 504.42
• Purity: ≥95%

BODIPY 581/591 C11 is a BODIPY borofluoroprene derivative with good light stability and low fluorescence artifacts. BODIPY 581/591 C11 can be used for study lipid peroxidation and antioxidant properties in living cells, or detect ferroptosis by reaction with hydroxyl radicals. BODIPY 581/591 C11 is emitted at 591 nm (reduced prototype), or redshifted to 510 nm (oxidized type). The excitation wavelengths were 581 nm (reduced prototype) and 500 nm (oxidized type)[1].
Preparation of the stock solution Dissolve 1mg BODIPY 581/591 C11 in 0.1983 mL DMSO to obtain 10 mM of BODIPY 581/591 C11. Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of BODIPY 581/591 C11 working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 2-10 μM of BODIPY 581/591 C11 working solution. Note: Please adjust the concentration of BODIPY 581/591 C11 working solution according to the actual situation. Cell staining 2.1 Cell preparation:For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. 2.2 Add 1 mL of BODIPY 581/591 C11 working solution, and then incubate at room temperature for 30 minutes. 2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. 2.4 Wash twice with PBS, 5 minutes each time. 2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer. Precautions 1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles. 2. Please adjust the concentration of BODIPY 581/591 C11 working solution according to the actual situation. 3. This product is for R&D use only, not for drug, household, or other uses. 4. For your safety and health, please wear a lab coat and disposable gloves to operate.

Reference

[1]. Gregor P C Drummen, et al. C11-BODIPY(581/591), an oxidation-sensitive fluorescent lipid peroxidation probe: (micro)spectroscopic characterization and validation of methodology. Free Radic Biol Med. 2002 Aug 15;33(4):473-90.
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