For research use only. Not for therapeutic Use.
Br-DAPI is a marker dye in DAPI series. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells[1]. Storage: Keep away from light.
General Protocol 1. Preparation of DAPI working solution 1.1 Preparation of the stock solution Dissolve 1 mg DAPI in 1 mL ddH2O to obtain 1 mg/mL of stock solution. Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of DAPI working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 ug/mL of working solution. Note: Please adjust the concentration of DAPI working solution according to the actual situation. 2. Cell staining 2.1 Suspension cells（6-well plate） a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL. b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes. c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. d. Wash twice with PBS, 5 minutes each time. e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes. d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry. Storage -20°C, 1 year. Protect from light Precautions 1. Please adjust the concentration of DAPI working solution according to the actual situation. 2. This product is for R&D use only, not for drug, household, or other uses. 3. For your safety and health, please wear a lab coat and disposable gloves to operate.
| Catalog Number | I044988 |
| CAS Number | 2387906-44-5 |
| Synonyms | 3-bromo-2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide |
| Molecular Formula | C16H14BrN5 |
| Purity | ≥95% |
| InChI | InChI=1S/C16H14BrN5/c17-13-11-6-5-10(16(20)21)7-12(11)22-14(13)8-1-3-9(4-2-8)15(18)19/h1-7,22H,(H3,18,19)(H3,20,21) |
| InChIKey | GSBXNZFEZFIMCS-UHFFFAOYSA-N |
| SMILES | C1=CC(=CC=C1C2=C(C3=C(N2)C=C(C=C3)C(=N)N)Br)C(=N)N |
| Reference | [1]. Kapuscinski J, et al. DAPI: a DNA-specific fluorescent probe. Biotech Histochem. 1995 Sep;70(5):220-33. |
