For research use only. Not for therapeutic Use.
BRD7586 is a cell-permeable small-molecule inhibitor of SpCas9. BRD7586 is the smallest known anti-CRISPR[1].
BRD7586 specifically engages SpCas9 but not Cas12a in cells[1].
BRD7586 (0-20 μM; 24 h) dose-dependently inhibits SpCas9 in the HiBiT-knock-in assay in HEK293T cells[1].
BRD7586 (20 μM; 48 h) enhances the ratio of on-target versus off-target in HEK293T cells[1].
| Catalog Number | I041983 |
| CAS Number | 895460-70-5 |
| Synonyms | 3-(4-chlorophenyl)sulfonyl-N-(4-pyridin-4-yl-1,3-thiazol-2-yl)propanamide |
| Molecular Formula | C17H14ClN3O3S2 |
| Purity | ≥95% |
| InChI | InChI=1S/C17H14ClN3O3S2/c18-13-1-3-14(4-2-13)26(23,24)10-7-16(22)21-17-20-15(11-25-17)12-5-8-19-9-6-12/h1-6,8-9,11H,7,10H2,(H,20,21,22) |
| InChIKey | MLSIIIMAQNQEOV-UHFFFAOYSA-N |
| SMILES | C1=CC(=CC=C1S(=O)(=O)CCC(=O)NC2=NC(=CS2)C3=CC=NC=C3)Cl |
| Reference | [1]. Lim D, et al. A general approach to identify cell-permeable and synthetic anti-CRISPR small molecules. Nat Cell Biol. 2022 Dec;24(12):1766-1775. |
