Bromosulfalein

• CAT Number: I023728
• CAS Number: 71-67-0
• Molecular Formula: C20H8Br4Na2O10S2
• Molecular Weight: 837.99
• Purity: ≥95%
• Synonyms: Bromosulfalein, Bromosulfophthalein, Bromosulphalein, Sulfobromophthalein, Sulphobromophthalein
• Solubility: Soluble in DMSO
• Appearance: Solid powder
• Storage: Dry, dark and at 0 - 4℃ for short term (days to weeks) or -20℃ for long term (months to years).
• IUPAC Name: disodium;2-hydroxy-5-[4,5,6,7-tetrabromo-1-(4-hydroxy-3-sulfonatophenyl)-3-oxo-2-benzofuran-1-yl]benzenesulfonate
• InChI: InChI=1S/C20H10Br4O10S2.2Na/c21-15-13-14(16(22)18(24)17(15)23)20(34-19(13)27,7-1-3-9(25)11(5-7)35(28,29)30)8-2-4-10(26)12(6-8)36(31,32)33;;/h1-6,25-26H,(H,28,29,30)(H,31,32,33);;/q;2*+1/p-2
• InChIKey: GHAFORRTMVIXHS-UHFFFAOYSA-L
• SMILES: O=C1OC(C2=CC(S([O-])(=O)=O)=C(O)C=C2)(C3=CC(S([O-])(=O)=O)=C(O)C=C3)C4=C(Br)C(Br)=C(Br)C(Br)=C41.[Na+].[Na+]

Bromosulfalein (Cat.No:I023728) has been commonly used as both a substrate and inhibitor of organic anionic transporting polypeptide 1B1 (OATP1B1), OATP1B3, OATP1A2, and OATP2B1, as well as multidrug resistance protein 2 (MDR2). It has also been used for in vivo studies of liver and biliary system function and as an anionic dye for the quantitative estimation of protein levels in tissue homogenates or purified samples.

Reference

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2. Izumi, S., Nozaki, Y., Komori, T., et al. Substrate-dependent inhibition of organic anion transporting polypeptide 1B1: Comparative analysis with prototypical probe substrates estradiol-17β-glucuronide, estrone-3-sulfate, and sulfobromophthalein. Drug Metab. Dispos. 41(10), 1859-1866 (2013).
3. Karlgren, M., Vildhede, A., Norinder, U., et al. Classification of inhibitors of hepatic organic anion transporting polypeptides (OATPs): Influence of protein expression on drug-drug interactions. J. Med Chem. 55(10), 4740-4763 (2012).
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8. Oh, J.H., Park, S.E., Shim, C.K., et al. Biliary clearance of bromosulfophthalein in anesthetized and freely moving conscious rat. Biopharm. Drug. Dispos. 30(2), 94-98 (2009).
9. McGuire, J.C., Taylor, P., and Greene, L.A. A modified bromosulfalein assay for the quantitative estimation of protein. Anal. Biochem. 83(1), 75-81 (1977).