BTB-1

• CAT Number: I005100
• CAS Number: 86030-08-2
• Molecular Formula: C12H8ClNO4S
• Molecular Weight: 297.71
• Purity: ≥95%
• Synonyms: BTB1; BTB 1 ; BTB-1.;4-chloro-2-nitro-1-(phenylsulfonyl)benzene
• Target: Cytoskeleton
• Solubility: Soluble in DMSO, not in water
• Storage: 0 - 4 °C for short term or -20 °C for long term
• IUPAC Name: 1-(benzenesulfonyl)-4-chloro-2-nitrobenzene
• InChI: InChI=1S/C12H8ClNO4S/c13-9-6-7-12(11(8-9)14(15)16)19(17,18)10-4-2-1-3-5-10/h1-8H
• InChIKey: VZDUQPHKUBZMLW-UHFFFAOYSA-N
• SMILES: C1=CC=C(C=C1)S(=O)(=O)C2=C(C=C(C=C2)Cl)[N+](=O)[O-]

BTB-1(CAT: I005100) is a novel small-molecule inhibitor of Kif18A, a mitotic motor protein essential for proper chromosome alignment during cell division. Identified through a high-throughput screen by Catarinella et al., it inhibits Kif18A’s microtubule (MT)-stimulated ATPase activity with an IC₅₀ of 1.7–1.9 µM. BTB-1 is highly selective within the kinesin subgroup, showing minimal effects on related proteins like Kif4, Eg5, and CENP-E. It acts as an ATP-competitive and MT-uncompetitive inhibitor, specifically targeting ATPase activity of the MT-bound Kif18A and reversibly blocking its MT gliding ability. This compound is particularly valuable in cell cycle and cancer research, serving as a tool for elucidating Kif18A’s roles in mitosis and exploring its therapeutic potential in targeting mitotic dysfunctions.