BTB09089

• CAT Number: I041747
• CAS Number: 245728-44-3
• Molecular Formula: C14H12Cl2N4OS2
• Molecular Weight: 387.31
• Purity: ≥95%
• Synonyms: 3-[(2,4-dichlorophenyl)methylsulfanyl]-1,6-dimethylpyridazino[4,5-e][1,3,4]thiadiazin-5-one
• InChI: InChI=1S/C14H12Cl2N4OS2/c1-19-11-6-17-20(2)13(21)12(11)23-14(18-19)22-7-8-3-4-9(15)5-10(8)16/h3-6H,7H2,1-2H3
• InChIKey: PIAWQVCBQLTEIY-UHFFFAOYSA-N
• SMILES: CN1C2=C(C(=O)N(N=C2)C)SC(=N1)SCC3=C(C=C(C=C3)Cl)Cl

BTB09089 is a T cell death-associated gene 8 (TDAG8/GPR65) specific agonist. BTB09089 increases TDAG8 expression and regulates the cytokine production of T cells and macrophages[1].
BTB09089 (0-18 μM; 30 min) significantly increases cAMP accumulation in hTDAG8 and mTDAG8 transiently expressing HEK293 cells, but not in control HEK 293 cells. BTB09089 does not increase cAMP accumulation in hGPR4 expressing HEK293 cells nor does it increase inositol phosphate accumulation in hOGR1 expressing HEK293 cells[1].
BTB09089 (0-18 μM; 30 min) significantly enhances the cAMP accumulation in a dose-dependent manner at pH 7.0-7.9 but not at pH 6.5[1].
BTB09089 (1-5 μM; 20 h) suppresses IL-2 production in splenocytes from WT mice in a dose-dependent manner but not from TDAG8 KO mice without affecting the cell viability[1].
BTB09089 (1-5 μM; 18 h) suppresses LPS (HY-D1056)-stimulated TNF-α and IL-6 production, and enhances LPS-stimulated IL-10 production in thioglycollate induced peritoneal exude cells (TG-PEC)[1].
BTB09089 (5-20 μM, 8 μL; i.c.v.; 6 hours prior to MCAO) reduces cerebral ischaemia-reperfusion injury in rats[2].

Reference

[1]. Onozawa Y, et al. Activation of T cell death-associated gene 8 regulates the cytokine production of T cells and macrophages in vitro. Eur J Pharmacol. 2012 May 15;683(1-3):325-31.
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[2]. Ma XD, et al. TDAG8 activation attenuates cerebral ischaemia-reperfusion injury via Akt signalling in rats. Exp Neurol. 2017 Jul;293:115-123.
 [Content Brief]