CA-074 methyl ester

• CAT Number: I001812
• CAS Number: 147859-80-1
• Molecular Formula: C19H31N3O6
• Molecular Weight: 397.50
• Purity: ≥95%
• Synonyms: methyl (2S)-1-[(2S)-3-methyl-2-[[(2S,3S)-3-(propylcarbamoyl)oxirane-2-carbonyl]amino]pentanoyl]pyrrolidine-2-carboxylate
• Target: Cathepsin
• Solubility: 10 mM in DMSO
• Storage: Store at -20°C
• IUPAC Name: methyl (2S)-1-[(2S,3S)-3-methyl-2-[[(2S,3S)-3-(propylcarbamoyl)oxirane-2-carbonyl]amino]pentanoyl]pyrrolidine-2-carboxylate
• InChI: InChI=1S/C19H31N3O6/c1-5-9-20-16(23)14-15(28-14)17(24)21-13(11(3)6-2)18(25)22-10-7-8-12(22)19(26)27-4/h11-15H,5-10H2,1-4H3,(H,20,23)(H,21,24)/t11-,12-,13-,14-,15-/m0/s1
• InChIKey: XGWSRLSPWIEMLQ-YTFOTSKYSA-N
• SMILES: CCCNC(=O)[C@@H]1[C@H](O1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N2CCC[C@H]2C(=O)OC

CA-074 methyl ester (Cat No.:I001812) is a cell-permeable inhibitor of cathepsin B, a lysosomal cysteine protease. It is derived from the parent compound CA-074, but the addition of the methyl ester group enhances its cell permeability. CA-074 methyl ester selectively inhibits cathepsin B activity and has been widely used in research to investigate the role of cathepsin B in various biological processes. By inhibiting cathepsin B, CA-074 methyl ester can modulate cellular processes such as apoptosis, autophagy, inflammation, and tumor progression. Its specific inhibition of cathepsin B makes it a valuable tool for understanding the functions of this protease in both normal physiological and pathological conditions.

Reference

[1]. Xu Y, et al. Protective mechanisms of CA074-me (other than cathepsin-B inhibition) against programmed necrosis induced by global cerebral ischemia/reperfusion injury in rats. Brain Res Bull. 2016 Jan;120:97-105.

[2]. Patel N, et al. CA-074Me compound inhibits osteoclastogenesis via suppression of the NFATc1 and c-FOS signaling pathways. J Orthop Res. 2015 Oct;33(10):1474-86.

[3]. Zhang L, et al. Treatment with CA-074Me, a Cathepsin B inhibitor, reduces lung interstitial inflammation and fibrosis in a rat model of polymyositis. Lab Invest. 2015 Jan;95(1):65-77.

[4]. Cho K, et al. CA-074Me, a cathepsin B inhibitor, decreases APP accumulation and protects primary rat cortical neurons treated with okadaic acid. Neurosci Lett. 2013 Aug 26;548:222-7.