CA-5f

• CAT Number: I019471
• CAS Number: 1370032-19-1
• Molecular Formula: C₂₄H₂₄N₂O₃
• Molecular Weight: 388.46
• Purity: ≥95%
• Target: Autophagy; Apoptosis
• Storage: Store at -20°C
• IUPAC Name: (3E,5E)-3-[(3,4-dimethoxyphenyl)methylidene]-5-(1H-indol-3-ylmethylidene)-1-methylpiperidin-4-one
• InChI: InChI=1S/C24H24N2O3/c1-26-14-18(10-16-8-9-22(28-2)23(11-16)29-3)24(27)19(15-26)12-17-13-25-21-7-5-4-6-20(17)21/h4-13,25H,14-15H2,1-3H3/b18-10+,19-12+
• InChIKey: JYOLPDWVAMBMQN-UBIAKTOFSA-N
• SMILES: CN1CC(=CC2=CC(=C(C=C2)OC)OC)C(=O)C(=CC3=CNC4=CC=CC=C43)C1

CA-5f（Cat No.:I019471） is a powerful inhibitor of late macroautophagy/autophagy that functions by suppressing the fusion of autophagosomes with lysosomes. By inhibiting this crucial step, CA-5f disrupts the degradation process within cells. As a result, it increases the levels of LC3B-II, a marker for monitoring autophagy, and SQSTM1 protein. This disruption also leads to the promotion of reactive oxygen species (ROS) production.

Reference

[1]. Zhang L, et al. Identification of compound CA-5f as a novel late-stage autophagy inhibitor with potent anti-tumor effect against non-small cell lung cancer. Autophagy. 2019 Mar;15(3):391-406.